在这项对3岁小猎犬周边视网膜的免疫组织学研究中,切除的视网膜标本用抗巢蛋白抗体免疫染色,Oct4,Nanog,Sox2,CDX2,细胞角蛋白18(CK18),RPE65和YAP1,以及苏木精和DAPI,两个核污渍。我们的发现揭示了内部视网膜中各种大小的孤立囊肿。有趣的是,在小囊肿的腔中观察到大量具有少量细胞质的小圆形细胞,而许多杂乱无章的细胞部分占据了大囊肿的空腔。小囊肿的巢蛋白呈强烈阳性,Oct4,Nanog,Sox2、CDX2、CK18和YAP1。仅在囊肿周围的组织中观察到RPE65阳性细胞。由于RPE65是视网膜色素上皮(RPE)细胞的特异性标志物,周围囊肿的周围细胞可能来自于视网膜内迁移的RPE细胞。在小囊肿中,巢蛋白强烈阳性染色,视网膜干细胞的标志,似乎表明它们来自视网膜干细胞。胚泡和RPE细胞标记的形态和阳性染色表明小囊肿可能形成了类似胚泡的结构,可能是由视网膜干细胞和迁移的RPE细胞之间的相互作用引起的。
In this immunohistological study on the peripheral retina of 3-year-old beagle dogs, excised retina specimens were immunostained with antibodies against nestin, Oct4, Nanog, Sox2, CDX2, cytokeratin 18 (CK 18), RPE65, and YAP1, as well as hematoxylin and DAPI, two nuclear stains. Our findings revealed solitary cysts of various sizes in the inner retina. Intriguingly, a mass of small round cells with scant cytoplasms was observed in the cavity of small cysts, while many disorganized cells partially occupied the cavity of the large cysts. The small cysts were strongly positive for nestin, Oct4, Nanog, Sox2, CDX2, CK18, and YAP1. RPE65-positive cells were exclusively observed in the tissue surrounding the cysts. Since RPE65 is a specific marker of retinal pigment epithelial (RPE) cells, the surrounding cells of the peripheral cysts were presumably derived from RPE cells that migrated intraretinally. In the small cysts, intense positive staining for nestin, a marker of retinal stem cells, seemed to indicate that they were derived from retinal stem cells. The morphology and positive staining for markers of blastocyst and RPE cells indicated that the small cysts may have formed structures resembling the blastocyst, possibly caused by the interaction between retinal stem cells and migrated RPE cells.