■这项研究的目的是调查从鄱阳湖地区的三个地点收集的叮咬mid和蜱的细菌群落,即,群鲁实践基地,桃花花园,和黄通畜牧业,以及病媒是否携带任何可能导致人类疾病的细菌病原体,为前瞻性的病原发现和疾病防治提供科学依据。
■使用代谢组学方法与全长16SrRNA基因测序和可操作的系统发育单位(OPU)分析相结合,我们表征了两个重要媒介物种的物种级微生物群落结构,咬mid和蜱,包括33个节肢动物样本,包括3,885个人,收集在鄱阳湖周围。
■总共有662个OPU被归类为咬mid,包括195个已知物种和373个潜在的新物种,618个OPU被分类为蜱,包括217个已知物种和326个潜在的新物种。令人惊讶的是,在两种节肢动物载体中均检测到具有潜在致病性的OPU,据报道,有66种已知的叮咬mid携带潜在的病原体,包括Asaialannensis和立克次体bellii,与50只蜱相比,如不动杆菌和葡萄球菌。我们发现变形杆菌是mid和tick中最主要的群体。此外,研究结果表明,mid和tick的微生物群倾向于由一些高度丰富的细菌控制。Pantoeasp7在叮咬mid中占主导地位,而柯西拉sp1富含蜱。同时,柯希拉属。,这可能对长骨血丝Neumann的生存至关重要,在所有蜱样品中都检测到。在这项研究中,对叮咬mid和tick的优势物种和病原体的鉴定有助于扩大我们与节肢动物媒介微生物相关的知识。
■叮咬mid和tick携带大量已知和潜在的新型细菌,并携带广泛的潜在致病菌,这可能会对人类和动物造成感染的风险。mid和tick的微生物群落往往由一些高度丰富的细菌主导。
UNASSIGNED: The purpose of this study was to investigate the bacterial communities of biting midges and ticks collected from three sites in the Poyang Lake area, namely, Qunlu Practice Base, Peach Blossom Garden, and Huangtong Animal Husbandry, and whether vectors carry any bacterial pathogens that may cause diseases to humans, to provide scientific basis for prospective pathogen discovery and disease prevention and control.
UNASSIGNED: Using a metataxonomics approach in concert with full-length 16S rRNA gene sequencing and operational phylogenetic unit (OPU) analysis, we characterized the species-level microbial community structure of two important vector species, biting midges and ticks, including 33 arthropod samples comprising 3,885 individuals, collected around Poyang Lake.
UNASSIGNED: A total of 662 OPUs were classified in biting midges, including 195 known species and 373 potentially new species, and 618 OPUs were classified in ticks, including 217 known species and 326 potentially new species. Surprisingly, OPUs with potentially pathogenicity were detected in both arthropod vectors, with 66 known species of biting midges reported to carry potential pathogens, including Asaia lannensis and Rickettsia bellii, compared to 50 in ticks, such as Acinetobacter lwoffii and Staphylococcus sciuri. We found that Proteobacteria was the most dominant group in both midges and ticks. Furthermore, the outcomes demonstrated that the microbiota of midges and ticks tend to be governed by a few highly abundant bacteria. Pantoea sp7 was predominant in biting midges, while Coxiella sp1 was enriched in ticks. Meanwhile, Coxiella spp., which may be essential for the survival of Haemaphysalis longicornis Neumann, were detected in all tick samples. The identification of dominant species and pathogens of biting midges and ticks in this study serves to broaden our knowledge associated to microbes of arthropod vectors.
UNASSIGNED: Biting midges and ticks carry large numbers of known and potentially novel bacteria, and carry a wide range of potentially pathogenic bacteria, which may pose a risk of infection to humans and animals. The microbial communities of midges and ticks tend to be dominated by a few highly abundant bacteria.