Komagataellaphafii(K.phaffii)(巴斯德毕赤酵母),也被称为生物技术酵母,是一种在生物技术和制药行业中具有许多应用的酵母。这种甲基营养酵母作为生产重组蛋白的平台引起了极大的兴趣。许多益处包括促进异源蛋白的容易纯化的有效分泌表达,细胞密度高,生长迅速,翻译后的变化,和整合到基因组中的稳定基因表达。在过去的三十年里,K.phafii也已被完善为适应性细胞工厂,可以在实验室环境中和工业规模上生产数百种生物分子。的确,到目前为止,已经使用K.phafii表达方法产生了超过5000种重组蛋白,占总细胞蛋白的30%或总释放蛋白的80%。K.phafii已用于制造70多种商业产品,以及已获得许可的300多种工业过程。其中包括用于工业生物技术的有用酶,包括木聚糖酶,甘露聚糖酶,脂肪酶,和植酸酶。其他的是生物制药,其中包括人血清白蛋白,胰岛素,乙型肝炎表面抗原,和表皮生长因子.与其他表达系统相比,这种酵母也被认为是合成亚单位疫苗的特殊宿主,最近被替代疫苗类型所取代,例如灭活/灭活和减毒活疫苗。此外,通过多级优化方法实现重组蛋白的高效生产,如密码子偏倚,基因剂量,promotors,信号肽,和环境因素。因此,虽然K.phafii表达系统是有效和简单的,有明确的过程程序,仍然需要确定理想的条件,因为这些条件根据靶蛋白而变化,以确保最高的重组蛋白生成。这篇综述涉及K.phafii表达系统,它在工业和生物制药蛋白质生产中的重要性,以及一些有效生产蛋白质的生物加工和遗传修饰策略。K.phaffii最终将继续在研究领域和工业应用中作为有效的表达系统做出贡献。
Komagataella phaffii (K. phaffii) (Pichia pastoris), also called biotech yeast, is a yeast species with many applications in the
biotechnology and pharmaceutical industries. This methylotrophic yeast has garnered significant interest as a platform for the production of recombinant proteins. Numerous benefits include effective secretory expression that facilitates the easy purification of heterologous proteins, high cell density with rapid growth, post-translational changes, and stable gene expression with integration into the genome. In the last thirty years, K. phaffii has also been refined as an adaptable cell factory that can produce hundreds of biomolecules in a laboratory setting and on an industrial scale. Indeed, over 5000 recombinant proteins have been generated so far using the K. phaffii expression method, which makes up 30% of the total cell protein or 80% of the total released protein. K. phaffii has been used to manufacture more than 70 commercial products in addition to over 300 industrial processes that have been granted licenses. Among these are useful enzymes for industrial
biotechnology, including xylanase, mannanase, lipase, and phytase. The others are biopharmaceuticals, which include human serum albumin, insulin, hepatitis B surface antigen, and epidermal growth factor. Compared to other expression systems, this yeast is also considered a special host for synthesizing subunit vaccines, which have recently been supplanted by alternative vaccination types, such as inactivated/killed and live attenuated vaccines. Moreover, efficient production of recombinant proteins is achieved through multi-level optimization methods, such as codon bias, gene dosage, promoters, signal peptides, and environmental factors. Therefore, although K. phaffii expression systems are efficient and simple with clearly established process procedures, it is still necessary to determine the ideal conditions since these vary depending on the target protein to ensure the highest recombinant protein generation. This review addresses the K. phaffii expression system, its importance in industrial and biopharmaceutical protein production, and some bioprocessing and genetic modification strategies for efficient protein production. K. phaffii will eventually continue contributing as a potent expression system in research areas and industrial applications.