Lycogalaepidendrum是最广为人知的粘液菌之一,也是该群体中首次发现的代表。使用来自欧洲330个植物标本室标本的687个原始DNA序列,亚洲,北美和中美洲,澳大利亚,我们构建了Lycogala属的第一个详细系统发育,基于两个独立遗传的遗传标记,核糖体小亚基18SrRNA核基因(18SrDNA)和线粒体细胞色素氧化酶亚基I基因(COI)。在两个系统发育中,L.epidendrum似乎是一个多系群体,以众多分支为代表。该属的其他四个公认物种(L.confusum,L.conicum,L.exiguum,和L.flavofuscum)分散在对应的枝条之间。条形码间隙分析显示了60个18SrDNA系统组。它们彼此之间的距离不少于与Lycogala属的其他物种。对于18个具有18SrDNA和COI序列的系统组,分析了重组模式以测试生殖分离。与假设panmixis的模拟结果相反,没有发现核糖体和线粒体系统群之间的交叉,从而得出所有测试的系统组代表生物物种的结论。超过三分之一(39.6%)的研究标本共享一个18SrDNA系统群,我们认为是L.epidendrums.str.该组表现出最广泛的地理分布和最高的种内遗传变异性。几乎所有(93.3%)剩余的非单例18SrDNA系统群仅限于某些大陆甚至地区。同时,在给定位置,各种繁殖上孤立的系统群发生同胞。
Lycogala epidendrum is one of the most widely known myxomycete species and the first-ever discovered representative of this group. Using 687 original DNA sequences from 330 herbarium specimens from Europe, Asia, North and Central America, and Australia, we constructed the first detailed phylogenies of the genus Lycogala, based on two independently inherited genetic markers, the ribosome small subunit 18S rRNA nuclear gene (18S rDNA) and the mitochondrial cytochrome oxidase subunit I gene (COI). In both phylogenies, L. epidendrum appeared to be a polyphyletic group, represented by numerous clades. The four other recognized species of the genus (L. confusum, L. conicum, L. exiguum, and L. flavofuscum) are scattered between branches corresponding to L. epidendrum. A barcode gap analysis revealed 60 18S rDNA phylogroups of L. epidendrum, which are distant from each other not less than from other species of the genus Lycogala. For 18 of these phylogroups with both 18S rDNA and COI sequences available, recombination patterns were analyzed to test for reproductive isolation. In contrast to the results of a simulation assuming panmixis, no crossing between ribosomal and mitochondrial phylogroups was found, thus allowing the conclusion that all tested phylogroups represent
biospecies. More than one third (39.6%) of the studied specimens share a single 18S rDNA phylogroup, which we consider to be L. epidendrum s. str. This group displays the broadest geographic distribution and the highest intraspecific genetic variability. Nearly all (93.3%) of the remaining non-singleton 18S rDNA phylogroups are restricted to certain continents or even regions. At the same time, various reproductively isolated phylogroups occur sympatric at a given location.