■术后谵妄(POD)已成为一项严峻挑战,随着全球人口老龄化,其后果严重,发病率增加。然而,潜在的机制尚不清楚。我们的研究旨在探索术后谵妄行为的老年小鼠三个特定脑区和唾液中代谢物的变化,并确定潜在的非侵入性生物标志物。
■将18个月大的雄性C57/BL6小鼠随机分配到麻醉/手术组或对照组。在手术前24小时和手术后6、9和24小时进行行为测试。在海马中测量补体C3(C3)和S100钙结合蛋白B(S100β)水平,对唾液进行了代谢组学分析,海马体,皮质,和杏仁核样本.
■总共,在唾液中检测到43、33、38和14种差异代谢物,海马体,皮质,和杏仁核,分别。“丙酮酸”“α-亚麻酸”和“2-油酰基-1-棕榈酸-sn-甘油-3-磷酸胆碱”在一个共同途径中富集,可能是POD的潜在非侵入性生物标志物。在三个大脑区域观察到共同的变化,1-甲基组氨酸的上调和D-谷氨酰胺的下调。
■能量代谢障碍,氧化应激,和神经递质失调与POD的发展有关。确定唾液代谢物生物标志物水平的变化可能有助于开发POD的非侵入性诊断方法。
UNASSIGNED: Postoperative delirium (POD) has become a critical challenge with severe consequences and increased incidences as the global population ages. However, the underlying mechanism is yet unknown. Our study aimed to explore the changes in metabolites in three specific brain regions and saliva of older mice with postoperative delirium behavior and to identify potential non-invasive biomarkers.
UNASSIGNED: Eighteen-month-old male C57/BL6 mice were randomly assigned to the anesthesia/surgery or control group. Behavioral tests were conducted 24 h before surgery and 6, 9, and 24 h after surgery. Complement C3 (C3) and S100 calcium-binding protein B protein (S100beta) levels were measured in the hippocampus, and a metabolomics analysis was performed on saliva, hippocampus, cortex, and amygdala samples.
UNASSIGNED: In total, 43, 33, 38, and 14 differential metabolites were detected in the saliva, hippocampus, cortex, and amygdala, respectively. \"Pyruvate\" \"alpha-linolenic acid\" and \"2-oleoyl-1-palmitoy-sn-glycero-3-phosphocholine\" are enriched in one common pathway and may be potential non-invasive biomarkers for POD. Common changes were observed in the three brain regions, with the upregulation of 1-methylhistidine and downregulation of D-glutamine.
UNASSIGNED: Dysfunctions in energy metabolism, oxidative stress, and neurotransmitter dysregulation are implicated in the development of POD. The identification of changes in the level of salivary metabolite biomarkers could aid in the development of noninvasive diagnostic methods for POD.