■嗜水气单胞菌对淡水水产养殖特别有害,寻找噬菌体是一种有效的生物防治方法,但是在该领域很少有关于可能的温带噬菌体及其突变体的报道。在这项研究中,收集了与嗜水菌基因组中的噬菌体高度同源的毒性噬菌体,并进行了初步的生物学鉴定以了解其性质。
■取自福建鳗鱼池塘的水样,中国与菌株结合在一起。使用斑点试验方法和双层琼脂平板测定法进行确认和纯化。使用透射电子显微镜观察噬菌体病毒粒子。共68株气单胞菌。用于确定主机范围。制备1,000、100、10、1、0.1、0.01、0.001、0.0001、0.00001的MOI组以检测最佳MOI。热稳定性测定条件设置为30、40、50、60、70和80°C,持续1小时。分别,酸碱稳定性测定条件设定为pH2.0、4.0、6.0、8.0、10.0和12.0。MOI分别为0.01和0.1,设置以确定噬菌体的抑制能力。
■已从水产养殖水中分离出一种名为phiA051的新型毒性嗜水气单胞菌噬菌体。电镜观察表明,噬菌体phiA051由二十面体衣壳组成。噬菌体phiA051具有0.01的最佳感染复数(MOI),其爆发大小为108PFU/细胞。噬菌体在30-50°C或pH6.0-10.0的温度下保持高生存力1小时。噬菌体phiA051在爆发早期快速抑制病原体传播方面具有一定的潜力,它具有线性dsDNA,GC含量为60.55%,总长度为32,212bp,包括46个ORF。
■噬菌体phiA051表现为毒性噬菌体。然而,BLASTN结果显示,前25个命中中的23个是气单胞菌菌株的基因组。有人认为phiA051可能来自气单胞菌染色体中的某些原蛋白。进一步研究噬菌体phiA051如何从温带噬菌体转化为毒性噬菌体的机制,将为探索噬菌体的潜力提供独特的视角和思路。
UNASSIGNED: Aeromonas hydrophila is particularly harmful to freshwater aquaculture, and the search for phage is an effective biological control method, but reports of possible temperate phages and their mutants are rare in this field. In this study, a virulent phage highly homologous to prophage in the genomes of A. hydrophila was collected and preliminary biological characterization was carried out to understand its nature.
UNASSIGNED: Water samples taken from eel ponds in Fujian, China were combined with the strain. Spot test method and double-layer agar plate assay was used for confirmation and purification. Phage virions were observed using transmission electron microscope. A total of 68 strains of Aeromonas spp. were used to determine the host range. MOI groups of 1,000, 100, 10, 1, 0.1, 0.01, 0.001, 0.0001, 0.00001 were prepared to detect the optimal MOI. The conditions of thermal stability assay were set as 30, 40, 50, 60, 70 and 80°C for 1 h, respectively, and conditions of acid and alkali stability assay were set as 2.0, 4.0, 6.0, 8.0, 10.0 and 12.0 of pH. MOI of 0.01 and 0.1, respectively, are set to determine the inhibitory capacity of phage.
UNASSIGNED: A novel virulent A. hydrophila phage designated phiA051 has been isolated from aquaculture water. Electron microscopic observation showed that the phage phiA051 was composed of an icosahedral capsid. The phage phiA051 possesses an optimal multiplicity of infection (MOI) of 0.01, and its burst size was 108 PFU/cell. The phage maintained a high viability at temperatures of 30-50°C or pH 6.0-10.0 for 1 h. Phage phiA051 has certain potentials in rapidly inhibiting the spread of pathogen early in the outbreak, and it has a linear dsDNA with GC content of 60.55% and a total length of 32,212 bp, including 46 ORFs.
UNASSIGNED: The phage phiA051 behaved as a virulent phage. However, the BLASTN result showed that 23 of the top 25 hits were genomes of Aeromonas strains. It was suggested that phiA051 was probably derived from some prophage in the chromosome of Aeromonas. Further investigation of the mechanism how phage phiA051 transforms from a temperate phage to a virulent phage will provide a unique perspective and idea to explore the potential of prophages.