■这项研究评估了四种生物陶瓷根管封闭剂(RCS)在体内的细胞毒性。斑马鱼的胚胎特征,比如死亡率,生存,孵化,和一般形态,用作评估细胞毒性的参数。
■RCS,即GuttaFlowBioseal,MTAFillapex,CeraSeal生物陶瓷,和iRootSP,根据制造商的指导方针进行混合。通过将设定的RCS浸入E3溶液的1X稀释液中来制备提取液。然后,将提取液输送到培养皿中,在培养皿中允许斑马鱼胚胎发育.在受精后24、48、72和96小时评估细胞毒性。
■Kruskal-Wallis测试表明,除了GuttaFlowBioseal,死亡率,生存,其余三个生物陶瓷RCSs的斑马鱼胚胎孵化与阴性对照差异显著(P<0.05)。死亡率也有明显差异,生存,斑马鱼胚胎在GuttaFlowBioseal和其他三个RCS之间的孵化(P<0.05)。
■GuttaFlowBioseal的细胞毒性小于其他生物陶瓷RCS;MTAFillapex,CeraSeal生物陶瓷根管封闭剂,和iRootSP根管封闭剂表现出相当的细胞毒性。
UNASSIGNED: This study evaluated the cytotoxicity of four bioceramic root canal sealers (RCSs) in vivo. The embryonic zebrafish characteristics, such as mortality, survival, hatching, and general morphology, served as the parameters for assessing cytotoxicity.
UNASSIGNED: The RCSs, namely GuttaFlow Bioseal, MTA Fillapex, CeraSeal Bioceramic, and iRoot SP, were mixed according to the manufacturer\'s guidelines. The extract solution was prepared by immersing the set RCS into 1X dilution of E3 solution. Then, the extract solution was delivered into a Petri dish where zebrafish embryos were allowed to develop. Cytotoxicity was evaluated 24, 48, 72, and 96 hours after fertilization.
UNASSIGNED: The Kruskal-Wallis test showed that except for GuttaFlow Bioseal, the mortality, survival, and hatching of zebrafish embryos for the remaining three bioceramic RCSs were significantly different from the negative controls (P<0.05). Significant differences were also evident in the mortality, survival, and hatching of zebrafish embryos between GuttaFlow Bioseal and three other RCSs (P<0.05).
UNASSIGNED: GuttaFlow Bioseal was less cytotoxic than other bioceramics RCSs; MTA Fillapex, CeraSeal Bioceramic root canal sealer, and iRoot SP root canal sealer exhibited comparable cytotoxicity.