目的:评价腔内消毒剂葡萄糖酸氯己定(CHX)的抗菌效果,呃,Cr,YSGG激光器(ECL),和姜黄素光敏剂(CP)对乳酸菌和生物活性(BA)的剪切粘合强度(SBS)和散装填充复合材料(BFC)修复材料结合到龋齿受影响的牙本质(CAD)。
方法:在国际龋齿检测和评估系统(ICDAS)上分为4和5分的60颗人类下颌磨牙。用乳酸杆菌种接种样本后,根据使用的消毒方案(n=20),将所有样本任意分为三组。第1组和第2组:使用ECL的CAD消毒,第3组和第4组:使用CP进行CAD消毒,第5组和第6组:使用CHX的CAD消毒。空腔灭菌后,评估了存活率,并根据所使用的修复材料将每组进一步分为两个亚组.使用BFC修复材料修复第1、3和5组(n=10),和第2、4和6组(n=10)使用常规的块状填充树脂材料恢复。使用通用试验机(UTM)确定SBS,并在立体显微镜下检查脱粘表面以确定失效模式。Kruskal-Walis,方差分析,和PostHocTukey用于研究生存率和粘结强度值。
结果:ECL组乳杆菌存活率最高(0.73±0.13)。PDT激活CP的存活率最低(0.17±0.09)。第1组(ECL+BA)处理的样品表现出SBS的最大值(18.31±0.22MPa)。然而,第3组(CPBA)显示粘结强度的最小值(14.05±1.02MPa)。组间比较显示,第1组,第2组(ECLBFC)(18.11±0.14MPa),第5组(CHX+BA)(18.14±0.36MPa),和第6组(CHX+BFC)(18.18±0.35MPa)显示出相当的结合完整性结果(p>0.05)。
结论:用Er消毒的受龋齿影响的牙本质,Cr:YSGG,和氯己定改善生物活性和常规散装填充修复材料的粘合分数。
OBJECTIVE: To evaluate the antimicrobial effectiveness of cavity disinfectants chlorhexidine gluconate (CHX), Er, Cr, YSGG laser (ECL), and curcumin photosensitizer (CP) against Lactobacillus and shear bond strength (SBS) of Bioactive (BA) and bulk fill composite (BFC) restorative material bonded to carious affected dentin (CAD).
METHODS: Sixty human mandibular molars scored 4 and 5 on the International Caries Detection and Assessment System (ICDAS) were included. After inoculating the specimens with lactobacillus specie all the samples were arbitrarily divided into three groups based on the disinfection regime used (n=20). Groups 1 and 2: CAD disinfection using ECL, Groups 3 and 4: CAD disinfection using CP, and Groups 5 and 6: CAD disinfection using CHX. After cavity sterilization, the survival rate was estimated and each group was further divided into two sub-groups based on the restorative material used. Groups 1, 3, and 5 (n = 10) were restored using BFC restorative material, and groups 2, 4, and 6 (n = 10) were restored using a conventional bulk-fill resin material. A universal testing machine (UTM) was used to determine the SBS and debonded surfaces were examined under a stereomicroscope to determine the modes of failure. Kruskal-Walis, ANOVA, and Post Hoc Tukey were applied to investigate the survival rate and bond strength values.
RESULTS: ECL group displayed the highest survival rate (0.73±0.13) of Lactobacillus. The least survival rate (0.17±0.09) was demonstrated by CP activated by PDT. Group 1 (ECL+ BA) treated specimens exhibited the maximum value of SBS (18.31 ± 0.22 MPa). However, group 3 (CP + BA) revealed the minimum values of bond strength (14.05 ± 1.02 MPa). The intergroup comparison revealed that group 1, group 2 (ECL+BFC) (18.11 ± 0.14 MPa), group 5 (CHX+ BA) (18.14 ± 0.36 MPa), and group 6 (CHX+BFC) (18.18 ± 0.35 MPa) displayed comparable outcomes of bond integrity (p>0.05).
CONCLUSIONS: Caries-affected dentin disinfected with Er, Cr: YSGG, and chlorhexidine improve bond scores of bioactive and conventional bulk-fill restorative material.