在这里,制备了一种简易的大微孔聚己内酯/鸭足胶原支架(PCL/DC),并对其进行了表征,以证实其在骨组织工程中的适用性。用于骨组织工程和骨缺损再生的仿生支架是重要元素。PCL具有良好的生物相容性和生物可降解性,是一种广泛应用于骨组织工程的生物材料。然而,PCL的高疏水性和低细胞附着位点特性导致支架设计中的微环境不足。胶原蛋白是一种广泛用于组织工程的天然生物材料,具有优异的生物相容性,机械性能,和细胞附着部分。在可以获得胶原蛋白的资源中,DC含有大量的I型胶原(COL1),是生物相容的,并且具有成本效益。在这项研究中,通过将DC与PCL以不同比例混合并应用非溶剂诱导相分离(NIPS)和热诱导相分离(TIPS)(N-TIPS)来制造支架,溶剂浇铸和颗粒浸出(SCPL),气体发泡法制备大微孔结构。通过形态分析对制备的支架进行了表征,生物活性试验,物理化学分析,和机械测试。体外研究通过生存力测试进行,形态学观察,和基因表达。结果表明,DC的掺入增强了支架的物理化学和机械性能。此外,根据生物活性测试中的DC含量,形成了大量的骨模拟磷灰石。体外研究表明,PCL/DC支架具有生物相容性,磷灰石和DC的存在为细胞增殖和分化形成了有利的微环境。总的来说,新型多孔PCL/DC支架可作为骨组织工程和再生的生物材料模型。
Herein, a facile macro- and microporous polycaprolactone/duck\'s feet collagen scaffold (PCL/DC) was fabricated and characterized to confirm its applicability in bone tissue engineering. A biomimetic scaffold for bone tissue engineering and regeneration for bone defects is an important element. PCL is a widely applied biomaterial for bone tissue engineering due to its biocompatibility and biodegradability. However, the high hydrophobicity and low cell attachment site properties of PCL lead to an insufficient microenvironment in designing a scaffold. Collagen is a nature-derived biomaterial that is widely used in tissue engineering and has excellent biocompatibility, mechanical properties, and cell attachment moieties. Among the resources from which collagen can be obtained, DC contains a high amount of collagen type I (COL1), is biocompatible, and is cost-effective. In this study, the scaffolds were fabricated by blending DC with PCL in various ratios and applied non-solvent-induced phase separation (NIPS) and thermal-induced phase separation (TIPS) (N-TIPS), solvent casting and particulate leaching (SCPL), and gas foaming method to fabricate macro- and microporous structure. The characterization of the fabricated scaffolds was carried out by morphological analysis, bioactivity test, physicochemical analysis, and mechanical test. In vitro study was carried out by viability test, morphology observation, and gene expression. The results showed that the incorporation of DC enhances the physicochemical and mechanical properties of the scaffolds. Also, a large amount of bone mimetic apatite was formed according to the DC content in the bioactivity test. The in vitro study showed that the PCL/DC scaffold is biocompatible and the existence of apatite and DC formed a favorable microenvironment for cell proliferation and differentiation. Overall, the novel porous PCL/DC scaffold can be a promising biomaterial model for bone tissue engineering and regeneration.