在同时测量双层张力的情况下,显示了在胆固醇(30摩尔%)存在下脂质双层(二叶酰磷脂酰胆碱)的渗透性水渗透性数据。使用移动膜法评估水渗透性的详细方法和程序(K.Yano,M.岩本,T.Koshiji和S.Oiki:使用移动膜方法在测量的双层张力下可视化脂质双层的渗透水渗透率。膜科学杂志,627(2021)119231)。平面脂质双层在玻璃毛细管中形成,将两个具有不同渗透压的水性隔室分开,渗透驱动的水通量被可视化为沿毛细管的膜运动。在校正未搅拌层效应后,在恒定的膜面积和张力下评价透水性。在这些测量中,几何特征,例如平面脂质双层的边缘以及双层与单层之间的接触角,进行了图像分析。对未搅拌层进行电生理评估,在其中使用了小草菌素A通道。在存在渗透梯度的情况下,gramicidin通道产生的流媒体潜力,并且示出了测量的流动电势数据和导出的水-离子耦合比(水通量/离子通量)。移动膜的集成方法的详细描述使研究人员能够重现实验,并有机会检查各种类型膜的水渗透性,包括含有水通道蛋白的.将目前的渗透水渗透率数据与以前发表的数据进行比较,而他们忽略了双层的紧张关系。
Data of the osmotic water permeability of a lipid bilayer (diphytanoylphosphaticylcholin) in the presence of cholesterol (30 mole%) are shown under the simultaneous measurement of bilayer tension. Detailed methods and procedures for evaluating the water permeability using the moving membrane method (K. Yano, M. Iwamoto, T. Koshiji & S. Oiki: Visualizing the Osmotic Water Permeability of a Lipid Bilayer under Measured Bilayer Tension Using a Moving Membrane Method. Journal of Membrane Science, 627 (2021) 119231) are presented. The planar lipid bilayer is formed in a glass capillary, separating two aqueous compartments with different osmolarities, and osmotically-driven water flux is visualized as membrane movements along the capillary. The water permeability was evaluated under constant membrane area and tension after correcting for the unstirred layer effect. In these measurements, geometrical features, such as the edge of the planar lipid bilayer and the contact angle between bilayer and monolayer, were image-analyzed. The unstirred layer was evaluated electrophysiologically, in which gramicidin A channel was employed. In the presence of an osmotic gradient, the gramicidin channel generates the streaming potential, and the measured streaming potential data and the derived water-ion coupling ratio (water flux/ion flux) are shown. Detailed descriptions of the integrated method of the moving membrane allow researchers to reproduce the experiment and give opportunities to examine water permeability of various types of membranes, including those containing aquaporins. The present data of osmotic water permeability are compared with the previously published data, while they neglected the bilayer tension.