■有证据表明,GABA能系统和5-羟色胺再摄取抑制剂(SSRI)如西酞普兰都参与了焦虑和抑郁过程的调节。在这项研究中,我们研究了GABAA受体药物和西酞普兰对雄性小鼠焦虑和抑郁相关行为的影响及其相互作用。
■对于脑室内(i.c.v.)输注,在左侧脑室植入了引导套管.使用高架迷宫(EPM)和强迫游泳测试(FST)评估焦虑和抑郁行为。
■结果表明,i.c.v.显微注射麝香酚(1µg/小鼠)可提高EPM测试中的%OAT(开放臂时间)和%OAE(开放臂进入),并减少FST中的不动时间而不影响运动活动,在EPM和FST中表现出抗焦虑和抗抑郁样行为,分别。另一方面,i.c.v.微量注射双微果碱(1µg/小鼠)可降低%OAT和%OAE,而不影响运动活动和不动时间,表现出类似焦虑的效果。此外,i.p.给予西酞普兰(8mg/kg)增加了%OAT和%OAE,并减少了不动时间,对运动活动没有影响,在雄性小鼠中显示抗焦虑和抗抑郁样反应。此外,i.c.v.输注无效剂量的麝香酚增强了由i.p.在雄性小鼠中注射西酞普兰诱导的抗焦虑药和抗抑郁药样反应。当西酞普兰和双子胶共同注射时,在雄性小鼠中,非显着剂量的双微果碱逆转了西酞普兰的抗焦虑作用。此外,数据显示,在雄性小鼠中,西酞普兰和麝香酚之间具有协同的抗焦虑和抗抑郁样行为。
■结果表明西酞普兰和GABA能药物之间的相互作用对雄性小鼠的焦虑和抑郁行为的调节。
UNASSIGNED: There is evidence that both the GABAergic system and serotonin reuptake inhibitor (SSRI) such as citalopram are involved in the modulation of anxiety and depression processes. In this research, we examined the effects of GABAA receptor agents and citalopram on anxiety- and depression-related behaviors and their interaction in male mice.
UNASSIGNED: For intracerebroventricular (i.c.v.) infusion, a guide cannula was implanted in the left lateral ventricle. Anxiety and depression behaviors were evaluated using the elevated plus-maze (EPM) and forced swimming test (FST).
UNASSIGNED: The results revealed that i.c.v. microinjection of muscimol (1 µg/mouse) enhanced % OAT (open arm time) and % OAE (open arm entries) in the EPM test and decreased immobility time in the FST without affecting locomotor activity, presenting anxiolytic- and antidepressant-like behaviors in the EPM and FST, respectively. On the other hand, i.c.v. microinjection of
bicuculline (1 µg/mouse) reduced % OAT and % OAE without affecting locomotor activity and immobility time, presenting an anxiogenic-like effect. Moreover, i.p. administration of citalopram (8 mg/kg) increased %OAT and %OAE and reduced immobility time with no effect on locomotor activity, showing anxiolytic- and antidepressant-like responses in male mice. Furthermore, i.c.v. infusion of an ineffective dosage of muscimol potentiated the anxiolytic- and antidepressant-like responses induced by i.p. injection of citalopram in male mice. When citalopram and
bicuculline were co-injected, a non-significant dose of
bicuculline reversed the anxiolytic-like effect of citalopram in male mice. Also, the data revealed synergistic anxiolytic- and antidepressant-like behaviors between citalopram and muscimol in male mice.
UNASSIGNED: The results suggested an interaction between citalopram and GABAergic agents on the modulation of anxiety and depression behaviors in male mice.