背景:类胡萝卜素是具有生物学作用的脂溶性植物化学物质,包括皮肤中的紫外线保护功能。光谱皮肤类胡萝卜素测量还可以用作类胡萝卜素消耗的非侵入性生物标志物。代谢基因中的单核苷酸多态性(SNP)与人血浆类胡萝卜素浓度有关,然而,它们与皮肤类胡萝卜素浓度的关系是未知的。
目的:本研究的目的是确定富含类胡萝卜素的番茄汁干预前后13种候选SNP与皮肤和血浆类胡萝卜素浓度之间的关系。
方法:在本随机分组中,对照试验,参与者(n=80)提供了富含番茄红素的蔬菜汁,提供低(13.1毫克),培养基(23.9毫克),和高(31.0毫克)每日总类胡萝卜素剂量8周。通过HPLC测量血浆类胡萝卜素浓度,在基线和研究时间点结束时通过反射光谱法(VeggiMeter™)评估皮肤类胡萝卜素评分。使用基于PCR的测定从血液中对5个基因(BCO1、CD36、SCARB1、SETD7、ABCA1)中的13个候选SNP进行基因分型。混合模型测试了干预的效果,研究时间点,干预与研究时间点之间的相互作用,在整个研究中皮肤和血浆类胡萝卜素的SNP基因型。基线类胡萝卜素摄入量,BMI,性别,和年龄是所有模型中的协变量。
结果:当评估基线和干预的最后一周时,CD36rs1527479(p=0.0490)的基因型与皮肤类胡萝卜素浓度显着相关。BCO1rs7500996(p=0.0067)和CD36rs1527479(p=0.0018)的基因型是组合SNP模型中皮肤类胡萝卜素浓度的重要预测因子。
结论:在理解遗传变异如何影响人类皮肤类胡萝卜素表型的个体间变异的基础上,这些新的SNP和皮肤类胡萝卜素浓度之间的关联得以扩展。在Clinicaltrials.gov注册的临床试验试验标识符是NCT03202043。
BACKGROUND: Carotenoids are fat-soluble phytochemicals with biological roles, including ultraviolet protective functions in skin. Spectroscopic skin carotenoid measurements can also serve as a noninvasive biomarker for carotenoid consumption. Single-nucleotide polymorphisms (SNPs) in metabolic genes are associated with human plasma carotenoid concentrations; however, their relationships with skin carotenoid concentrations are unknown.
OBJECTIVE: The objective of this study was to determine the relationship between 13 candidate SNPs with skin and plasma carotenoid concentrations before and after a carotenoid-rich tomato juice intervention.
METHODS: In this randomized, controlled trial, participants (n = 80) were provided with lycopene-rich vegetable juice providing low (13.1 mg), medium (23.9 mg), and high (31.0 mg) daily total carotenoid doses for 8 wk. Plasma carotenoid concentrations were measured by high-pressure liquid chromatography, and skin carotenoid score was assessed by reflection spectroscopy (Veggie Meter) at baseline and the end-of-study time point. Thirteen candidate SNPs in 5 genes (BCO1, CD36, SCARB1, SETD7, and ABCA1) were genotyped from blood using PCR-based assays. Mixed models tested the effects of the intervention, study time point, interaction between intervention and study time point, and SNP genotype on skin and plasma carotenoids throughout the study. Baseline carotenoid intake, body mass index, gender, and age are covariates in all models.
RESULTS: The genotype of CD36 rs1527479 (P = 0.0490) was significantly associated with skin carotenoid concentrations when baseline and the final week of the intervention were evaluated. Genotypes for BCO1 rs7500996 (P = 0.0067) and CD36 rs1527479 (P = 0.0018) were significant predictors of skin carotenoid concentrations in a combined SNP model.
CONCLUSIONS: These novel associations between SNPs and skin carotenoid concentrations expand on the understanding of how genetic variation affects interindividual variation in skin carotenoid phenotypes in humans. This trial was registered at clinicaltrials.gov as NCT03202043.