一个绿色的,介绍了测定班布特罗及其主要降解产物的方法,特布他林,同时,依靠同步荧光技术。在Δλ=20nm处测量一阶导数同步荧光光谱振幅,所以班布特罗可以在260nm处定量,特布他林可以在290nm处测量,每个在另一个的过零点。班布特罗和特布他林的振幅-浓度图在0.2-6.0µgml-1和0.2-4.0µgml-1的浓度范围内呈线性关系,分别。遵循官方指南来计算所提出的方法的验证参数。班布特罗和特布他林的检出限低值为0.023,0.056µgml-1,定量限为0.071,0.169µgml-1,分别,指出该方法的灵敏度。班布特罗是特布他林的前药,而后者被认为是其降解产物,因此所建立的方法可以被视为稳定性指示方法。此外,该方法用于单成分制剂中班布特罗和特布他林的分析,结果与参考方法具有统计学一致性。建议的方法,作为一个简单和低成本的程序,优于以前发表的需要更复杂技术的方法,更长的分析时间和剧毒的溶剂和试剂。它可以被认为是一种生态友好的分析程序。
A green, sensitive and cost-effective method is introduced in this research for the determination of
bambuterol and its main degradation product, terbutaline, simultaneously, relying on the synchronous spectrofluorimetric technique. First derivative synchronous spectrofluorimetric amplitude is measured at Δλ = 20 nm, so
bambuterol can be quantitated at 260 nm, and terbutaline can be measured at 290 nm, each at the zero crossing point of the other. The amplitude-concentration plots were linear over the concentration ranges of 0.2-6.0 µg ml-1 and 0.2-4.0 µg ml-1 for both bambuterol and terbutaline, respectively. Official guidelines were followed to calculate the validation parameters of the proposed method. The low values of limits of detection of 0.023, 0.056 µg ml-1 and limits of quantitation of 0.071, 0.169 µg ml-1 for
bambuterol and terbutaline, respectively, point to the sensitivity of the method.
Bambuterol is a prodrug for terbutaline, and the latter is considered its degradation product so the established method could be regarded as a stability-indicating one. Moreover, the proposed method was used for the analysis of
bambuterol and terbutaline in their single ingredient preparations and the results revealed statistical agreement with the reference method. The suggested method, being a simple and low-cost procedure, is superior to the previously published methods which need more sophisticated techniques, longer analysis time and highly toxic solvents and reagents. It could be considered as an eco-friendly analytical procedure.