在动物粪便中发现的许多抗生素抗性基因(ARG)和毒力因子(VF)对人类健康构成重大风险。然而,石墨烯亚硒酸钠(GSSe)的影响,一种新的化学纳米硒,和生物纳米硒(BNSSe),一种新的生物强化纳米硒,细菌硒代谢,趋化性,ARGs,动物粪便中的VFs仍然未知。在这项研究中,我们采用高通量测序法研究了GSSe和BNSSe对肉鸡粪肥ARGs和VFs表达的影响。结果表明,BNSSe通过抑制细菌硒化合物代谢途径降低厌氧发酵过程中的硒压力,从而降低粪肥硒污染。此外,与亚硒酸钠和GSSe组相比,BNSSe组的ARGs和VFs的表达水平较低,因为BNSSe抑制细菌趋化途径。共现网络分析确定了以下PhylaBacteroidetes(Butyricimonas,Odoribacter,Paraprevotella,和Rikenella),Firmicutes(乳酸杆菌属,Candidatus_Borkfalkia,Merdimonas,镰刀菌,肠单胞菌,和Megamonas),和变形杆菌(脱硫弧菌属)。发现ARGs和VFs基因的表达和丰度与ARGs-VFs共存有关。此外,细菌硒化合物代谢和趋化性途径的BNSSe破坏导致ARG和VF的转移频率降低。这些发现表明BNSSe可以通过抑制细菌硒化合物代谢和趋化途径来降低动物粪便中ARGs和VFs的表达。
Numerous antibiotic resistance genes (ARGs) and virulence factors (VFs) found in animal manure pose significant risks to human health. However, the effects of graphene sodium selenite (GSSe), a novel chemical nano-Selenium, and biological nano-Selenium (BNSSe), a new bioaugmentation nano-Se, on bacterial Se metabolism, chemotaxis, ARGs, and VFs in animal manure remain unknown. In this study, we investigated the effects of GSSe and BNSSe on ARGs and VFs expression in broiler manure using high-throughput sequencing. Results showed that BNSSe reduced Se pressure during anaerobic fermentation by inhibiting bacterial selenocompound metabolism pathways, thereby lowering manure Selenium pollution. Additionally, the expression levels of ARGs and VFs were lower in the BNSSe group compared to the Sodium Selenite and GSSe groups, as BNSSe inhibited bacterial chemotaxis pathways. Co-occurrence network analysis identified ARGs and VFs within the following phyla Bacteroidetes (genera Butyricimonas, Odoribacter, Paraprevotella, and Rikenella), Firmicutes (genera Lactobacillus, Candidatus_Borkfalkia, Merdimonas, Oscillibacter, Intestinimonas, and Megamonas), and Proteobacteria (genera Desulfovibrio). The expression and abundance of ARGs and VFs genes were found to be associated with ARGs-VFs coexistence. Moreover, BNSSe disruption of bacterial selenocompound metabolism and chemotaxis pathways resulted in less frequent transfer of ARGs and VFs. These findings indicate that BNSSe can reduce ARGs and VFs expression in animal manure by suppressing bacterial selenocompound metabolism and chemotaxis pathways.