甜玉米已在世界范围内流行。传统的甜玉米具有低浓度的必需营养素,如赖氨酸(0.15-0.25%),色氨酸(0.03-0.04%)和维生素原A(proA3-4ppm),和缺乏导致人类严重的健康问题。这里,shrunken2(sh2)的堆叠,opaque2(o2),在四个杂种的亲本中进行了番茄红素ε环化酶(lcyE)和β-胡萝卜素羟化酶(crtRB1)基因。,APQH1,APHQ4,APHQ5和APHQ7使用标记辅助回交育种(MABB)。sh2的基因连锁标记(umc2276和umc1320),而o2的基于基因的标记(umc1066和phi057),lcyE(5'TE-InDel)和crtRB1(3'TE-InDel),用于BC1F1、BC2F1和BC2F2的基因分型。选定的回交后代显示出轮回亲本基因组的高恢复率(92.4-97.7%)。重组甜玉米杂交种具有显著高的赖氨酸(0.390%),色氨酸(0.082%)和proA(21.14ppm),加上高甜度(白利糖度18.96%)。改良的甜玉米杂交种在三种环境中具有较高的穗轴产量(12.22-15.33t/ha)。这些新开发的生物强化甜玉米杂交种在提供均衡营养方面具有重要意义。这是结合sh2,o2,lcyE和crtRB1基因富集具有多种必需营养素的甜玉米杂交种的首次报道。
Sweet corn has gained worldwide popularity. Traditional sweet corn possesses low concentration of essential nutrients such as lysine (0.15-0.25%), tryptophan (0.03-0.04%) and provitamin-A (proA 3-4 ppm), and deficiency leads to serious health problems in humans. Here, stacking of shrunken2 (sh2), opaque2 (o2), lycopene epsilon cyclase (lcyE) and β-carotene hydroxylase (crtRB1) genes were undertaken in the parents of four hybrids viz., APQH1, APHQ4, APHQ5 and APHQ7 using marker-assisted
backcross breeding (MABB). Gene-linked markers (umc2276 and umc1320) for sh2, while gene-based markers for o2 (umc1066 and phi057), lcyE (5\'TE-InDel) and crtRB1 (3\'TE-InDel), were used for genotyping in BC1F1, BC2F1 and BC2F2. Selected
backcross progenies showed high recovery of recurrent parent genome (92.4-97.7%). The reconstituted sweet corn hybrids possessed significantly high lysine (0.390%), tryptophan (0.082%) and proA (21.14 ppm), coupled with high kernel sweetness (brix 18.96%). The improved sweet corn hybrids had high cob yield (12.22-15.33 t/ha) across three environments. These newly developed biofortified sweet corn hybrids possess great significance in providing balanced nutrition. This is the first report of combining sh2, o2, lcyE and crtRB1 genes for enrichment of sweet corn hybrids with multiple essential nutrients.