近几十年来,益生菌已成为促进虾生长和免疫调节的可接受的水产养殖策略。本研究旨在通过60天的试验来评估巴西芽孢杆菌对凡纳滨对虾的影响。将南美白对虾乳杆菌(3±0.4g)分为四组,每组重复三次,并以0、1×107、1×108和1×109CFU/g的剂量饲喂等氮饮食。被定义为控制,G1、G2和G3组,分别。B.velezensis显著提高了生长,存活率,和南美白对虾的近体成分(P<0.05)。饲喂维氏芽孢杆菌饮食的所有组均显示消化酶显着增加(脂肪酶,淀粉酶,和蛋白酶),超氧化物歧化酶(SOD;G3),过氧化氢酶(CAT;G3,G2和G1),溶菌酶活性(G3和G2),免疫球蛋白M(IgM),杀菌活性BA%,碱性磷酸酶(AKP),酸性磷酸酶(ACP)与对照组比较(P<0.05)。丙二醛(MDA),甘油三酯,胆固醇,高密度脂蛋白(HDL),低密度脂蛋白(LDL),天冬氨酸转氨酶(AST),与对照组相比,饲粮组丙氨酸转氨酶(ALT)水平显着降低(P<0.05)。SOD(G3)的表达水平,LZM,和丝氨酸蛋白酶基因显著高于对照组(P<0.05)。这是第一个解决凡纳滨对虾LZM和丝氨酸蛋白酶基因表达影响的研究。南美白对虾乳杆菌饲喂的肝胰腺中的B和R细胞比对照组多。总之,B.velezensis是一种有前途的益生菌,可以安全地添加到1×109CFU/g的凡纳滨对虾的饮食中。它的应用对健康状况有积极的影响,存活率,营养价值,和南美白对虾的免疫力。
In recent decades, probiotics have become an acceptable aquaculture strategy for shrimp growth promotion and immune modulation. This study aimed to evaluate the effect of
Bacillus velezensis on Litopenaeus vannamei following a 60-day trial. L. vannamei (3 ± 0.4 g) were distributed into four groups with three replicates per group and fed an isonitrogenous diet supplemented with B. velezensis at 0, 1 × 107, 1 × 108, and 1 × 109 CFU/g, which were defined as the control, G1, G2, and G3 groups, respectively. B. velezensis significantly improved the growth, survival rate, and proximate body composition of L. vannamei (P < 0.05). All groups fed the B. velezensis diet showed significant increases in digestive enzymes (lipase, amylase, and protease), superoxide dismutase (SOD; G3), catalase (CAT; G3, G2, and G1), lysozyme activity (G3 and G2), immunoglobulin M (IgM), bactericidal activity BA%, alkaline phosphatase (AKP), and acid phosphatase (ACP) compared with the control group (P < 0.05). Malondialdehyde (MDA), triglycerides, cholesterol, high-density lipoprotein (HDL), low-density lipoprotein (LDL), aspartate aminotransferase (AST), and alanine aminotransferase (ALT) levels were significantly decreased in all groups fed B. velezensis diet compared with the control group (P < 0.05). The expression levels of SOD (G3), LZM, and serine proteinase genes were significantly higher in L. vannamei fed diets containing B. velezensis than in the control group (P < 0.05). This is the first study to address the effects of B. velezensis on the expression of the LZM and serine proteinase genes in L. vannamei. L. vannamei fed diet containing B. velezensis had more B and R cells in its hepatopancreas than did the control group. In conclusion, B. velezensis is a promising probiotic that can be safely added to the diet of L. vannamei with 1 × 109 CFU/g. Its application had a positive influence on the health status, survival rate, nutritional value, and immunity of L. vannamei.