背景:幼儿和老年人易感肺炎链球菌引起的侵袭性肺炎球菌病(IPD)。肺炎球菌蛋白特异性抗体对IPD具有保护作用;然而,对收购的速度知之甚少,成熟,并在一生中维持这些抗体。
方法:免疫球蛋白G(IgG)和IgA水平,贪婪,和/或对健康幼儿血清和唾液中肺炎球菌蛋白质组的特异性,成年人,和老年人,具有已知的运输状态,通过酶联免疫吸附测定(ELISA)和针对ΔcpsTIGR4的二维蛋白质印迹进行测量。
结果:11个月大的儿童,最年轻的年龄组测试,血清和唾液中肺炎球菌蛋白质组特异性IgG和IgA水平和亲和力最低,其次是24个月大的儿童,在成人组中进一步升高.在成人群体中,父母的血清和唾液IgG和IgA抗体水平最高。在儿童中,抗体水平和亲和力与日托出勤和兄弟姐妹的存在相关,冒充暴露和免疫的代理人。免疫优势模式在整个生命中略有不同。
结论:针对肺炎球菌蛋白质组的体液免疫是通过肺炎球菌暴露的多次发作获得的。在幼儿中低水平和低亲合力的抗蛋白质组抗体谱可能有助于他们的IPD易感性,而在总体抗蛋白组抗体丰富的老年人中,其他因素可能起作用。
BACKGROUND: Young children and older adults are susceptible for invasive pneumococcal disease (IPD) caused by Streptococcus pneumoniae. Pneumococcal protein-specific antibodies play a protective role against IPD; however, not much is known about the pace of acquisition, maturation, and maintenance of these antibodies throughout life.
METHODS: Immunoglobulin G (IgG) and IgA levels,
avidity, and/or specificity to the pneumococcal proteome in serum and saliva from healthy young children, adults, and older adults, with known carriage status, were measured by enzyme-linked immunosorbent assay (ELISA) and 2-dimensional western blotting against ΔcpsTIGR4.
RESULTS: Eleven-month-old children, the youngest age group tested, had the lowest pneumococcal proteome-specific IgG and IgA levels and
avidity in serum and saliva, followed by 24-month-old children and were further elevated in adult groups. Among adult groups, the parents had the highest serum and saliva IgG and IgA antibody levels. In children, antibody levels and
avidity correlated with daycare attendance and presence of siblings, posing as proxy for exposure and immunization. Immunodominance patterns slightly varied throughout life.
CONCLUSIONS: Humoral immunity against the pneumococcal proteome is acquired through multiple episodes of pneumococcal exposure. Low-level and low-
avidity antiproteome antibody profiles in young children may contribute to their IPD susceptibility, while in overall antiproteome antibody-proficient older adults other factors likely play a role.