这项研究的目的是评估改性的细菌纤维素是否可用于人造耳廓骨骼。
背景:耳廓是外耳的一部分。它由皮肤组成,软骨,肌肉和脂肪组织。软骨使结构成形。重建有几个迹象,比如先天性异常,机械伤害,和烧伤,并且已经提出了一系列方法来完全重建耳廓。
方法:细菌纤维素膜,厚度至少为25毫米,通过木醋杆菌培养在体外产生。将整个人造人耳廓骨骼按比例制作,以使其植入动物模型-Wistar大鼠中。将40只大鼠分成4组,每组10只动物。每组分配不同的切除时间:14天,30天,90天或720天。每次切除后,对人造骨骼及其周围组织进行了检查。外科手术基于Nagata技术。
结果:在14、30、90和720天后进行的切除显示了人工骨骼在动物体内的愈合过程和整合的进展。骨架的形状或结构没有变化的迹象。
结论:在过去的几年中,已经开发了几种手术技术和生物技术方法来改善面部重建的结果。其他方法可用于创建耳廓软骨,基于支架和软骨细胞。
结论:我的研究结果表明,改性细菌纤维素可用于形成有效的人工耳廓,以保持其形状和弹性,没有退化的迹象。
The aim of this study was to assess whether modified bacterial cellulose can be used for an artificial auricle skeleton.
BACKGROUND: The auricle is a part of the external ear. It consists of skin, cartilage, muscles and adipose tissue. The cartilage gives shape to the structure. There are several indications for reconstruction, such as congenital anomalies, mechanical injuries, and burns, and a range of methods have been proposed for the complete reconstruction of the auricle.
METHODS: A bacterial cellulose membrane, at least 25 mm in thickness, was produced in vitro by Acetobacter xylinum culture. The entire artificial human auricle skeleton was made to scale to allow its implantation into an animal model - Wistar rats. Forty rats were divided into four groups of 10 animals. Each group was assigned a different resection time: 14 days, 30 days, 90 days or 720 days. After each resection, an examination of the artificial skeleton and the tissues surrounding it was conducted. The surgical procedure was based on the Nagata technique.
RESULTS: Resection after 14, 30, 90 and 720 days shows the progression of the healing process and integration of the artificial skeleton into the animal body. There are no signs of change in the shape or structure of the skeleton.
CONCLUSIONS: Several surgical techniques and biotechnological methods have been developed over the past few years to improve the results of facial reconstruction. Other approaches can be used to create auricle cartilage, based on scaffolds and chondrocytes.
CONCLUSIONS: My findings indicate that modified bacterial cellulose can be used to form an effective artificial auricle which appears to maintain its shape and elasticity, with no signs of degradation.