■本研究旨在通过在钛上施加双层羟基磷灰石(HA)氧化锆(ZrO2)涂层来评估成骨细胞和抗菌活性,从而确认这两种材料的协同作用。
■本研究中使用的标本分为四组:对照组(抛光钛;T组)和三个实验组:TH组(射频磁控溅射HA沉积钛),Z组(ZrO2ALD沉积钛),和组ZH(RF磁控溅射HA和ZrO2ALD沉积钛)。变形链球菌(S.突变体)使用结晶紫测定法评估表面。附着力,扩散,和MC3T3-E1细胞的分化,小鼠成骨细胞系,通过WST-8测定和ALP测定进行评估。
■组Z显示变形链球菌的粘附力降低(p<0.05)和成骨细胞活力提高(p<0.0083)。TH组和ZH组显示变形链球菌的粘附减少(p<0.05),成骨细胞增殖和细胞分化增加(p<0.0083)。ZH组表现出最高的抗菌和成骨细胞分化。
■总之,沉积在钛上的双层HA和ZrO2在抑制变形链球菌的粘附方面更有效,诱导生物膜形成,并通过两种材料的协同作用增加参与骨整合的成骨细胞分化。
UNASSIGNED: This study aimed to confirm the synergy effect of these two materials by evaluating osteoblast and antibacterial activity by applying a double-layered hydroxyapatite(HA) zirconium oxide(ZrO2) coating to titanium.
UNASSIGNED: The specimens used in this study were divided into four groups: a control group (polished titanium; group T) and three experimental groups: Group TH (RF magnetron sputtered HA deposited titanium), Group Z (ZrO2 ALD deposited titanium), and Group ZH (RF magnetron sputtered HA and ZrO2 ALD deposited titanium). The adhesion of Streptococcus mutans (S.mutans) to the surface was assessed using a crystal violet assay. The adhesion, proliferation, and differentiation of MC3T3-E1 cells, a mouse osteoblastic cell line, were assessed through a WST-8 assay and ALP assay.
UNASSIGNED: Group Z showed a decrease in the adhesion of S. mutans (p < 0.05) and an improvement in osteoblastic viability (p < 0.0083). Group TH and ZH showed a decrease in adhesion of S. mutans (p < 0.05) and an increase in osteoblastic cell proliferation and cell differentiation (p < 0.0083). Group ZH exhibited the highest antibacterial and osteoblastic differentiation.
UNASSIGNED: In conclusion double-layered HA and ZrO2 deposited on titanium were shown to be more effective in inhibiting the adhesion of S. mutans, which induced biofilm formation, and increasing osteoblastic differentiation involved in osseointegration by the synergistic effect of the two materials.