洛基栖息地,全球分布的生态系统,拥有多样化的生物群,包括许多特有和濒危物种。在这些环境中茁壮成长的维管植物面临着具有挑战性的非生物条件,需要不同的形态和生理适应。他们与周围微生物组的接触是,然而,对他们的适应同样重要,健身,和长期生存。然而,在这个迷人的生物生态系统中,围绕这种复杂的相互作用仍然缺乏理解。使用显微镜观察和元编码分析,我们检查了居住在岩石中的西喀尔巴厘特有灌木的根系中的真菌丰度和多样性,达芙妮刺槐(百草科)。我们探索了根相关真菌群落的多样化与研究地点的微气候变化有关。我们揭示了归因于不同生态行会的多种分类真菌群体对达芙妮根的广泛定植,主要是植物病原体,深色隔内生菌(DSE),和丛枝菌根真菌(AMF)。值得注意的是,在较冷和较温暖的微环境之间出现了分类组成和生态行会的差异。除了无所不在的AMF,温暖的地方表现出植物病原体的流行,而较冷的地点的特点是DSE占主导地位。这种真菌生物多样性,很可能是由环境引发的,这表明,在温暖地区的D.arbuscula种群可能更容易受到真菌疾病的影响,特别是在全球气候变化的背景下。
Rocky habitats, globally distributed ecosystems, harbour diverse biota, including numerous endemic and endangered species. Vascular plants thriving in these environments face challenging abiotic conditions, requiring diverse morphological and physiological adaptations. Their engagement with the surrounding microbiomes is, however, equally vital for their adaptation, fitness, and long-term survival. Nevertheless, there remains a lack of understanding surrounding this complex interplay within this fascinating biotic ecosystem. Using microscopic observations and metabarcoding analyses, we examined the fungal abundance and diversity in the root system of the rock-dwelling West Carpathian endemic shrub, Daphne arbuscula (Thymelaeaceae). We explored the diversification of root-associated fungal communities in relation to microclimatic variations across the studied sites. We revealed extensive colonization of the Daphne roots by diverse taxonomic fungal groups attributed to different ecological guilds, predominantly plant pathogens, dark septate endophytes (DSE), and arbuscular mycorrhizal fungi (AMF). Notably, differences in taxonomic composition and ecological guilds emerged between colder and warmer microenvironments. Apart from omnipresent AMF, warmer sites exhibited a prevalence of plant pathogens, while colder sites were characterized by a dominance of DSE. This mycobiome diversification, most likely triggered by the environment, suggests that D. arbuscula populations in warmer areas may be more vulnerable to fungal diseases, particularly in the context of global climate change.