压力,在生物体中引发许多生理和行为反应,是导致抑郁症和焦虑症等精神疾病发展的重要风险因素。本研究旨在探讨炎症,氧化应激状态,焦虑,以及暴露于慢性间歇性冷应激的青少年啮齿动物的抑郁样行为。青春期雄性大鼠接受改良的慢性间歇性冷应激模型(21天,1小时/天,4°C)。使用蔗糖偏好和强迫游泳测试评估抑郁样行为,虽然焦虑样行为是使用开放领域评估的,高架加上迷宫,和明暗盒子测试。我们测量了皮质醇水平,肿瘤坏死因子-α,白细胞介素-1β,脑源性利钠因子,活性氧,丙二醛,总氧化剂和抗氧化剂,和前额叶皮层中的其他化学物质,丘脑,纹状体,用ELISA和比色法检测大鼠海马脑区。数据采用Studentt检验和Pearson相关分析。冷应激治疗后,受试者的焦虑和抑郁样行为都显著增加。我们的研究揭示了暴露于压力的受试者中各个大脑区域的显着变化。冷应激导致前额叶皮层和纹状体的BDNF水平降低(p<0.05),前额叶皮质皮质醇水平升高(p<0.05),海马和丘脑中IL-1β水平升高(p<0.05),纹状体中蛋白质羰基水平增加(p<0.05),前额叶皮质和丘脑的TAS降低(p<0.05)。暴露于寒冷的青春期大鼠表现出焦虑和抑郁样行为。这项研究观察到不同大脑区域的炎症和氧化应激增加,然而,对压力的反应各不相同。我们的研究结果表明,青春期是一个对压力敏感性增强的时期,这可能会导致戏剧性的后果。
Stress, which triggers numerous physiological and behavioral responses in the organism, is a significant risk factor that contributes to the development of psychiatric disorders such as depression and anxiety. This study aimed to investigate the inflammation, oxidative stress status, anxiety, and depression-like behaviors of adolescent rodents exposed to chronic intermittent cold stress. Adolescent male rats were subjected to a modified chronic intermittent cold stress model (21 days, 1 hour/day, 4 °C). Depression-like behaviors were evaluated using the sucrose preference and forced swimming tests, while anxiety-like behaviors were assessed using the open field, elevated plus maze, and light-dark box tests. We measured levels of cortisol, tumor necrosis factor-α, interleukin-1β, brain-derived natriuretic factor, reactive oxygen species, malondialdehyde, total oxidants and antioxidants, and other chemicals in the prefrontal cortex, thalamus, striatum, and hippocampus brain regions of rats using ELISA and colorimetric methods. Data were analyzed using Student\'s t-test and Pearson correlation analysis. After the cold stress treatment, both anxiety and depression-like behaviors increased remarkably in the subjects. Our study revealed significant changes in various brain regions among the stress-exposed subjects. Cold stress resulted in decreased BDNF levels in the prefrontal cortex and striatum (p < 0.05), increased cortisol levels in the prefrontal cortex (p < 0.05), increased IL-1β levels in the hippocampus and thalamus (p < 0.05), increased protein carbonyl levels in the striatum (p < 0.05), and decreased TAS in the prefrontal cortex and thalamus (p < 0.05). Adolescent rats exposed to cold exhibit both anxiety- and depression-like behaviors. This study observed an increase in inflammation in various brain regions, yet the responses to stress varied. Our findings suggest that adolescence is a period of heightened sensitivity to stress, which can lead to dramatic consequences.