本文介绍了通过生产短链脂肪酸和诱导白细胞介素12对抗肿瘤特性的体外评估。此外,它提供了24种gasseri乳杆菌的最重要和功能性益生菌特性,植物乳杆菌,嗜酸乳杆菌,和从人类中分离出的发酵Limosilactum菌株,食物,和发酵食品。为此,在酸性环境(pH=2.5)中生存,对胆汁盐的耐受性,在胃蛋白酶-胰酶存在下的活力,附着力百分比,抗生素耐药性,自动聚合,并研究了与三种人类肠道病原体接触的潜在共聚集百分比。这些病原体是大肠杆菌O157:H7NCTC12900,肠沙门氏菌亚种。肠ATCC13076和单核细胞增生李斯特菌ATTC7644。此外,研究了小鼠脾细胞中IL-12的体外诱导量,以评估19株gasseri和植物乳杆菌的抗肿瘤特性,以及5株发酵乳杆菌和嗜酸乳杆菌的短链脂肪酸(SCFA)的发展。采用气相色谱火焰离子化检测器(GC-FID)和酶联免疫吸附测定(ELISA)分别测定短链脂肪酸和IL-12。所有菌株在酸性条件下都具有高活力。在LF56,LF57,LF55,OF,和F和菌株分别为LF56、LF57和A7。除LF56外,所有菌株均对胆汁盐具有高抗性。L.gasseri54C在19个菌株中具有最高的平均粘附分数(疏水性),为62.9%。尽管植物乳杆菌的不同菌株对所测试的抗生素的敏感性,M8和M11,S2G,A7,LF55,LF57和5G对卡那霉素和氯霉素具有抗性,分别。此外,21G对氨苄青霉素耐药,LF56对四环素和M8,而M11,LF56和21G对红霉素。此外,L.gasseri对氨苄青霉素表现出中度抗性,红霉素,和四环素,而发酵乳杆菌ATCC9338对氨苄青霉素表现出良好的抗性,红霉素,和氯霉素.在这方面,植物乳杆菌LF56和gasseri54C对三种病原菌的平均自聚集和共聚集最高,分别。最高和最低水平的短链脂肪酸乙酸分别由从Horre41.62分离的发酵乳杆菌19SH和从发酵种子27.047分离的发酵乳杆菌21SH产生。此外,L.发酵菌,具有传统发酵食品来源的OF代码,产生最多的异丁酸,丁酸,和戊酸,值为0.6828、0.74165和0.49915mmol,分别。从人类来源分离的具有代码F的发酵乳杆菌产生的异戊酸最多,为1.1874mmol。所有测试菌株均从发酵种子中产生良好的丙酸,但发酵乳杆菌21SH除外。在菌株中,从牛奶中分离的植物乳杆菌M11和从人类中分离的L.gasseri52B对IL-12的体外诱导最高,这可能与其细胞壁组成和结构有关。
This paper presents an in vitro evaluation of antitumor properties through producing short-chain fatty acids and inducing interleukin 12. In addition, it offers the most important and functional probiotic properties of 24 Lactobacillus gasseri, Lactiplantibacillus plantarum, Lactobacillus acidophilus, and Limosilactobacillus fermentum strains isolated from humans, foods, and fermented foods. To this end, survival in an acidic environment (pH = 2.5), tolerance in bile salt, viability in the presence of pepsin-pancreatin, adhesion percentage, antibiotic resistance, auto-aggregation, and potential percentage of co-aggregation are studied in contact with three human intestinal pathogens. These pathogens are Escherichia coli O157: H7 NCTC 12900, Salmonella enterica subsp. enterica ATCC 13076, and Listeria monocytogenes ATTC 7644. Also, in vitro induction amount of IL-12 in mouse splenocytes is investigated to evaluate antitumor properties by 19 strains of L. gasseri and L. plantarum along with the development of short-chain fatty acids (SCFA) by 5 strains of L. fermentum and L. acidophilus. Gas Chromatography Flame Ionization Detector (GC-FID) and enzyme-linked immunosorbent assay (ELISA) were used to measure short-chain fatty acids and IL-12, respectively. All strains had high viability under acidic conditions. The highest levels of pancreatin and pepsin resistance were found in strains LF56, LF57, LF55, OF, and F and strains LF56, LF57, and A7, respectively. All strains except LF56 had high resistance to bile salts. L. gasseri 54C had the highest average adhesion score (hydrophobicity) of 62.9 % among 19 strains. Despite the susceptibility of different strains of L. plantarum to the tested antibiotics, M8 and M11, S2G, A7, LF55, LF57, and 5G were resistant to kanamycin and chloramphenicol, respectively. Also, 21G was resistant to ampicillin, LF56 to tetracycline and M8, and M11, LF56, and 21G to Erythromycin. In addition, L. gasseri showed moderate resistance to ampicillin, erythromycin, and tetracycline, while L. fermentum ATCC 9338 showed good resistance to ampicillin, erythromycin, and chloramphenicol. In this respect, L. plantarum LF56 and gasseri 54C had the highest average auto-aggregation and co-aggregation against three pathogenic bacteria, respectively. The highest and lowest levels of acetic acid as short-chain fatty acids were produced by L. fermentum 19SH isolated from Horre 41.62 and L. fermentum 21SH from fermented seeds 27.047, respectively. Moreover, L. fermentum, with the OF code of traditional-fermented food origin, produced the most isobutyric acid, butyric acid, and valeric acid, with values of 0.6828, 0.74165, and 0.49915 mmol, respectively. L. fermentum isolated from the human origin with code F produced the most isovaleric acid of 1.1874 mmol. All the tested strains produced good propionic acid except L. fermentum 21SH from fermented seeds. Among strains, L. plantarum M11 isolated from milk and L. gasseri 52B from humans had the highest in vitro induction of IL-12, which is probably related to their cell wall compositions and structure.