斑块状牛皮癣是一种慢性炎症性皮肤病,可引起鳞片覆盖的红色发炎病变。白细胞,包括树突状细胞和T细胞,通过产生多种细胞因子参与皮肤炎症,从而促进角质形成细胞的过度增殖。缺乏有效性和毒副作用是常规治疗的主要问题,涉及新的抗银屑病分子的研究是必不可少的。在这项研究中,两种天然多酚的抗炎和抗增殖作用,根皮素和巴沙酮C,使用T细胞和银屑病角质形成细胞的共培养进行了研究。根皮素通过调节抗原Ki67和增殖细胞核抗原(PCNA)的表达发挥抗增殖活性。这些效果与甲氨蝶呤相当,中重度银屑病的参考治疗方法。有了balsaconeC,Ki67的表达也降低。此外,根皮素降低多种促炎细胞因子的水平:单核细胞趋化蛋白-1(MCP-1/CCL2),巨噬细胞炎性蛋白-1α(MIP-1α),粒细胞集落刺激因子(G-CSF),粒细胞-巨噬细胞集落刺激因子(GM-CSF),白细胞介素-1α(IL-1α),白细胞介素-1β(IL-1β),白细胞介素-6(IL-6),白细胞介素-17A(IL-17A),和肿瘤坏死因子α(TNF-α)。使用根皮素和甲氨蝶呤的白细胞介素-2(IL-2)水平的增加也代表了抗炎活性。巴沙酮C和甲氨蝶呤降低IL-1α和IL-1β水平,但甲氨蝶呤的降低幅度更高。总之,根皮素的抗炎作用比甲氨蝶呤和巴拉塞C更明显。使用根皮素或甲氨蝶呤后,淋巴细胞共同抗原(CD45)的表达与健康状态更为相似。最后,根皮素从其他化合物中脱颖而出,似乎有希望用于牛皮癣治疗。
Plaque psoriasis is a chronic inflammatory skin disease causing red inflamed lesions covered by scales. Leukocytes, including dendritic cells and T cells, participate in the inflammation of the skin by producing multiple cytokines, thus contributing to the hyperproliferation of keratinocytes. Lack of effectiveness and toxic side effects are the main concerns with conventional treatments, and research involving new antipsoriatic molecules is essential. In this study, the anti-inflammatory and antiproliferative effects of two natural polyphenols, phloretin and balsacone C, were investigated using the coculture of T cells and psoriatic keratinocytes. Phloretin exerted antiproliferative activity by regulating the expression of antigen Ki67 and proliferating cell nuclear antigen (PCNA). These effects were comparable to those of methotrexate, a reference treatment for moderate to severe psoriasis. With balsacone C, the expression of Ki67 was also reduced. Additionally, phloretin decreased the levels of multiple pro-inflammatory cytokines: monocyte chemoattractant protein-1 (MCP-1/CCL2), macrophage inflammatory protein-1α (MIP-1α), granulocyte colony-stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-1 alpha (IL-1α), interleukin-1 beta (IL-1β), interleukin-6 (IL-6), interleukin-17A (IL-17A), and tumor necrosis factor alpha (TNF-α). The increased interleukin-2 (IL-2) levels with phloretin and methotrexate also represented anti-inflammatory activity. Balsacone C and methotrexate decreased the levels of IL-1α and IL-1β, but methotrexate exerted a higher reduction. In summary, the anti-inflammatory effects of phloretin were more pronounced than those of methotrexate and balsacone C. In addition, the expression of lymphocyte common antigen (CD45) was more similar to that of the healthy condition after using phloretin or methotrexate. Finally, phloretin stood out from the other compounds and appears promising for psoriasis treatment.