黄芩(L.)R.Br.是一种药用植物,长期以来一直在传统医学中用于治疗脓肿等疾病,溃疡,耳朵和眼睛感染。已知它具有广泛的生物学特性,如抗菌,抗氧化剂,抗真菌药,抗炎,抗糖尿病和抗癌作用。在这项研究中,我们从侧柏的地上部分和根部建立了体外培养物。随后,我们比较了获得的提取物的基本植物化学特征,并进行了生物学分析,以评估其诱导乳腺癌(MCF-7)和肺癌(A549)细胞凋亡的潜力。通过HPLC-MS进行的植物化学分析显示存在属于酚酸的化合物(阿魏酸,注射器,香草味,迷迭香,绿原,咖啡因,coumaric,二羟基苯甲酸),黄酮类化合物(叶黄素醇和cirsimaritin),和萜烯,如6,11,12,14,16-五羟基-3,17二乙酰基-8,11,13-abietatrien-7-酮,6,11,12,14,16-五羟基-3,17-二乙酰基5,8,11,13-松香-7-酮,和3,6,12-三羟基-2-乙酰基-8,12-abietadien7,11,14-三酮。结果表明,两种提取物对MCF-7和A549癌细胞都具有细胞毒性和基因毒性作用,具有不同程度的敏感性。还表明,两种提取物都可以通过改变凋亡基因的表达来诱导凋亡(Bax,Bcl-2,TP53,Fas,和TNFSF10),降低线粒体膜电位,增加ROS水平,增加DNA损伤。此外,它已经表明,测试提取物可以改变血液凝固参数。我们的结果表明,从黄芩属植物地上部分和根的体外培养物中提取的提取物具有很好的治疗应用前景,但是需要进一步的研究来更好地了解它们在体外模型中的作用机制。
Plectranthus scutellarioides (L.) R.Br. is a medicinal plant that has long been used in traditional medicine to treat conditions such as abscesses, ulcers, and ear and eye infections. It is known to have a wide range of biological properties, such as antibacterial, antioxidant, antifungal, anti-inflammatory, anti-diabetic and anti-cancer effects. In this study, we established in vitro cultures from both the aerial parts and roots of Plectranthus scutellarioides. Subsequently, we compared the basic phytochemical profile of the obtained extracts and conducted a biological analysis to assess their potential for inducing apoptosis in breast (MCF-7) and lung (A549) cancer cells. Phytochemical analysis by HPLC-MS revealed the presence of compounds belonging to phenolic acids (ferulic, syringic, vanillic, rosmarinic, chlorogenic, caffeic, coumaric, dihydroxybenzoic acids), flavonoids (eriodyctiol and cirsimaritin), and terpenes such as 6,11,12,14,16-Pentahydroxy-3,17diacetyl-8,11,13-abietatrien-7-one, 6,11,12,14,16-Pentahydroxy-3,17-diacetyl5,8,11,13-abietatetraen-7-one, and 3,6,12-Trihydroxy-2-acetyl-8,12-abietadien7,11,14-trione. The results show that both extracts have a cytotoxic and genotoxic effect against MCF-7 and A549 cancer cells, with a different degree of sensitivity. It was also shown that both extracts can induce apoptosis by altering the expression of apoptotic genes (Bax, Bcl-2, TP53, Fas, and TNFSF10), reducing mitochondrial membrane potential, increasing ROS levels, and increasing DNA damage. In addition, it has been shown that the tested extracts can alter blood coagulation parameters. Our results indicate that extracts from in vitro cultures of Plectranthus scutellarioides aerial parts and roots have promising therapeutic application, but further research is needed to better understand the mechanisms of their action in the in vitro model.