疟疾是一种致命的威胁生命的寄生虫感染,是发病率和死亡率的主要原因。本研究的目的是评估简单的,便宜,准确,可靠,易于获得更好的诊断,可在医疗点(POC)快速检测疟疾。该研究包括从患者身上收集的1403个样本,其中1227例为临床疑似病例,176例为连续发热患者。在疑似病例中,通过PCR,只有338个样本被确认为阳性,889个样本为疟原虫物种阴性。所有889个样本通过显微镜显示疟原虫物种的阴性结果,疟疾Ag快速试剂盒,但只有867个样品用疟疾Ab快速试剂盒证实为阴性。在338份PCR阳性样本中,337个样本通过显微镜和疟疾Ag快速试剂盒证实为阳性,但只有284个样本使用疟疾抗体快速试剂盒被证实为阳性。总体而言,显微镜和基于疟疾抗原的侧流测定显示出相似的灵敏度,特异性,PPV,净现值和效率,分别,而PCR检测有100%的灵敏度,特异性,PPV,净现值和效率。但是疟疾抗体侧流测定的进化数据具有92.81%的灵敏度,94.13%的特异性,84.02%PPV,97.52%的净现值和93.80%的效率。开发的疟疾pf/pv抗原和抗体现场部署试剂盒是简单的,快速,准确,可靠,便宜,用户友好,POC。此外,试剂盒是高度敏感的和物种特异性的。发现pf/pv抗原试剂盒比疟疾pf/pv抗体快速试剂盒更准确,具有99.7%的灵敏度和100%的特异性。在开发的两种快速试剂盒中,发现疟疾pf/pv抗原试剂盒比疟疾pf/pv抗体快速试剂盒更准确,具有99.7%的灵敏度和100%的特异性。
Malaria is a fatal life-threatening parasitic infection and a leading cause of morbidity and mortality. The present study was aimed to evaluate simple, inexpensive, accurate, reliable, easily available better diagnostic for rapid detection of malaria at point of care (POC). The study includes 1403 samples collected from the patients, of which 1227 were clinically suspected cases and 176 from consecutive feverish patients. Among the suspected cases only 338 samples were confirmed positive and 889 samples were negative for Plasmodium species by PCR. All the 889 samples showed negative result for plasmodium species by microscopy, Malarial Ag rapid kits but only 867 samples were confirmed negative with malarial Ab rapid kits. Of the 338 PCR positive samples, 337 samples were confirmed positive by microscopy and Malarial Ag rapid kits, but only 284 samples were confirmed positive using malarial Ab rapid kits. Overall the microscopy and the malaria antigen-based lateral flow assay exhibited similar sensitivity, specificity, PPV, NPV and efficiency, respectively, whereas the PCR assay had 100% sensitivity, specificity, PPV, NPV and efficiency. But the evolutionary data for malaria antibody lateral flow assay has 92.81% sensitivity, 94.13% specificity, 84.02% PPV, 97.52% NPV and 93.80% efficiency. The developed Malaria pf/pv antigen and antibody field-deployable kits are simple, rapid, accurate, reliable, inexpensive, user friendly, POC. In addition the kits are highly sensitive and species-specific. The pf/pv antigen kit is found to be more accurate with 99.7% sensitivity and 100% specificity than to Malaria pf/pv antibody rapid kits. Of the two rapid kits developed, Malaria pf/pv antigen kit is found be more accurate with 99.7% sensitivity and 100% specificity than to Malaria pf/pv antibody rapid kits.