■本研究旨在评估椰子油提取物(COE)在实验性感染了新城疫病毒(vNDV)的肉鸡中的免疫调节作用。
■总共150只肉鸡(日龄)被平均分为五个研究组,即阴性对照,阳性对照,COE-1、COE-2和COE-3。在第10天,COE-1,COE-2和COE-3组的肉鸡分别每升饮用水补充1,2和3mlCOE,持续15天。在第13天,将0.1ml/鸟(10-5.25ELD50)的vNDV接种到阳性对照的肉鸡中,COE-1、COE-2和COE-3组肌内注射。在这项研究中,增长业绩,发病率,记录每个研究组的死亡率。在第7、14、21、28和35天测定抗NDV的抗体滴度。7日也测定了IgY和IgM的水平,14日,和SRBC接种后第21天。在第33天,在左侧的第一和第二脚趾之间(皮内)注射禽结核菌素以测量淋巴增生反应。在第35天,通过将炭黑墨水注射到右翼静脉中,通过碳清除率测定来评估血液中的吞噬活性。还收集具有大体损伤的内脏器官用于组织病理学。
■COE显着提高了增长绩效,降低了肉鸡的发病率和死亡率。在补充了COE的肉鸡中,针对NDV的抗体滴度以及针对SRBC的IgY和IgM抗体水平显着升高。淋巴增殖反应和吞噬活性也增强。在COE补充的群体中,COE-3组肉鸡的生长性能显着提高,免疫防御增强。
■椰子油提取物具有提高肉鸡生长性能和免疫状态的潜力。它可以有效地用作饲料添加剂和抗生素的替代品,以防止传染性家禽病原体的传播。
UNASSIGNED: This study aims to evaluate the immunomodulatory effects of coconut oil extract (COE) in broilers experimentally infected with velogenic Newcastle disease virus (vNDV).
UNASSIGNED: A total of 150 broiler birds (day-old) were equally divided into five study groups i.e., negative control, positive control, COE-1, COE-2, and COE-3. On day 10, broilers of groups COE-1, COE-2, and COE-3 were supplemented with 1, 2, and 3 ml of COE respectively per liter of drinking water for 15 days. On day 13, 0.1ml/bird (10-5.25 ELD50) of vNDV was inoculated in broilers of positive control, COE-1, COE-2, and COE-3 groups intramuscularly. During this study, growth performance, morbidity, and mortality rates of each study group were recorded. The antibody titer against NDV was determined on days 7, 14, 21, 28, and 35. The levels of IgY and IgM were also determined on the 7th, 14th, and 21st days post-SRBC inoculation. On day 33, avian tuberculin was injected between the 1st and 2nd toes of the left side (intradermally) to measure lymphoproliferative responses. On day 35, the phagocytic activity in the blood was assessed through a carbon clearance assay by injecting carbon black ink into the right-wing vein. The visceral organs having gross lesions were also collected for histopathology.
UNASSIGNED: The COE significantly improved the growth performance, and lowered the morbidity and mortality rates of broilers. There was a significant rise in antibody titers against NDV and levels of IgY and IgM antibodies against SRBC in COE-supplemented broilers. The lymphoproliferative response and phagocytic activity were also enhanced. Among COE-supplemented groups, the broilers of the COE-3 group showed a significant increase in growth performance and boosted immune defense.
UNASSIGNED: Coconut oil extract has the potential to boost the growth performance and immune status of broilers. It can be used effectively as a feed additive and alternative to antibiotics to prevent the spread of infectious poultry pathogens.