目的:本研究旨在提供体外实验证据,证明野生蘑菇具有用作药物的潜力,可有效对抗各种类型的癌症。
背景:纵观人类历史,除了食物,从蘑菇中获得的传统药物和天然毒药已被用于治疗许多疾病。显然,食用和药用蘑菇制剂具有有益的健康作用,没有已知的严重不良副作用。
目的:本研究旨在揭示五种不同食用蘑菇的细胞生长抑制潜力,并首次在此显示zonarius的生物活性。
方法:将蘑菇子实体干燥和粉末,然后用己烷提取,乙酸乙酯,和甲醇。通过自由基清除活性(DPPH)方法筛选蘑菇提取物的抗氧化活性。体外研究了提取物对A549(人肺癌)的抗增殖活性和细胞毒性,HeLa(人宫颈癌),HT29(人结肠癌),Hep3B(人肝癌),MCF7(人类乳腺癌),人羊膜细胞,和Beas2B(正常人细胞)细胞系通过使用MTT细胞增殖试验,乳酸脱氢酶(LDH)测定,DNA降解,TUNEL,和细胞迁移试验。
结果:使用增殖,细胞毒性,DNA降解,TUNEL,和迁移测定,我们展示了己烷,乙酸乙酯,和乳酸的甲醇提取物,硫脲Laetiporussulfureus,腹膜炎,猪苓,即使在低剂量(<45.0-99.6µg/mL)下,罗马利亚黄对细胞也是有效的,通过抑制迁移的方式,作为凋亡的负诱导剂。还证明了具有高抗氧化作用的蘑菇提取物在高于60μg/mL的浓度下对细胞膜具有20%-30%的可接受的细胞毒性活性。
结论:总体而言,所有具有高抗氧化作用的蘑菇提取物都具有很强的抗增殖活性和对细胞的低毒性。这些发现,至少,强调这些蘑菇提取物可用于治疗癌症疾病,特别是作为结肠的支持性疗法,肝脏,还有肺癌.
This study aims to provide in vitro experimental evidence that wild mushrooms have the potential to be used as a pharmaceutical that could be effective against various types of cancer.
Throughout human history, besides food, traditional medicine and natural poisons obtained from mushrooms have been used for the treatment of many diseases. Clearly, edible and medicinal mushroom preparations have beneficial health effects without the known severe adverse side effects.
This study was designed to reveal the cell growth inhibitory potential of five different edible mushrooms and the biological activity of Lactarius zonarius was shown here for the first time.
The mushrooms fruiting bodies were dried and powdered then extracted with hexane, ethyl acetate, and methanol. The mushroom extracts were screened for possible antioxidant activities by the free radical scavenging activity (DPPH) method. Antiproliferative activity and cytotoxicity of the extracts were investigated in vitro on A549 (human lung carcinoma), HeLa (human cervix carcinoma), HT29 (human colon carcinoma), Hep3B (human hepatoma), MCF7 (human breast cancer), FL (human amnion cells), and Beas2B (normal human cells) cells lines by using MTT cell proliferation assay, lactate dehydrogenase (LDH) assay, DNA degradation, TUNEL, and cell migration assay.
Using proliferation, cytotoxicity, DNA degradation, TUNEL, and migration assay, we displayed that hexane, ethyl acetate, and methanol extracts of the Lactarius zonarius, Laetiporus sulphureus, Pholiota adiposa, Polyporus squamosus, and Ramaria flava were effective on the cells even so at low doses (< 45.0 - 99.6 µg/mL) by acting in a way that represses migration, as a negative inducer of apoptosis. It was also demonstrated that mushroom extracts with high antioxidant effect have within the acceptable cytotoxic activity of 20%-30% on the cell membrane at concentrations higher than 60 µg/mL. Overall, all of the mushroom extracts with high antioxidant effects had strong antiproliferative activity and low toxicity for cells. These findings, at least, highlight that these mushroom extracts can be used for the treatment of cancer disease, especially as a supportive therapy against colon, liver, and lung cancer.