将生物细胞与人造传感器杂交能够以高特异性检测宽范围的弱生理反应。眼前房(ACE)由于其眼免疫特权和光学透明性而成为理想的移植部位。这使得能够对细胞和微组织进行优越的非侵入性纵向分析。ACE中生物混合微结构的参与可能,然而,受瞳孔反应和动力学的影响。这里,生物混合微结构的无缝线移植,IP-Visio光敏树脂3D打印,提出了含有精确定位的胰岛的小鼠ACE。生物混合微结构允许机械固定在ACE中,与虹膜动力学无关。移植后,尽管与血管源(虹膜)物理分离并浸入具有连续循环和清除的低粘度液体(房水)中,但微结构中的胰岛仍成功维持其功能超过20周,并血管化。这种方法为ACE中的生物混合微组织移植开辟了新的前景,推进微组织-宿主相互作用的监测,疾病建模,治疗结果,和工程组织中的血管化。本文受版权保护。保留所有权利。
Hybridizing biological cells with man-made sensors enable the detection of a wide range of weak physiological responses with high specificity. The anterior chamber of the eye (ACE) is an ideal transplantation site due to its ocular immune privilege and optical transparency, which enable superior noninvasive longitudinal analyses of cells and microtissues. Engraftment of biohybrid microstructures in the ACE may, however, be affected by the pupillary response and dynamics. Here, sutureless transplantation of biohybrid microstructures, 3D printed in IP-Visio photoresin, containing a precisely localized pancreatic islet to the ACE of mice is presented. The biohybrid microstructures allow mechanical fixation in the ACE, independent of iris dynamics. After transplantation, islets in the microstructures successfully sustain their functionality for over 20 weeks and become vascularized despite physical separation from the vessel source (iris) and immersion in a low-viscous liquid (aqueous humor) with continuous circulation and clearance. This approach opens new perspectives in biohybrid microtissue transplantation in the ACE, advancing monitoring of microtissue-host interactions, disease modeling, treatment outcomes, and vascularization in engineered tissues.