目的:本研究旨在确定细菌性阴道病(BV)的患病率,阴道毛滴虫(TV)共感染,以及细菌分离株的抗菌敏感性。
方法:该研究是对2019年4月至9月期间进行常规产前检查的232名孕妇进行的横断面研究。2020年,在拉各斯的阿穆科科诊所,尼日利亚。妇科医生对每位患者进行了临床检查,以寻找阴道分泌物及其一致性/均匀性,颜色和气味。从每位患者中抽取两个阴道拭子(HVS)样本,并使用半结构化问卷收集社会人口统计信息,实践/态度,和每个参与者的临床信息。使用一个样品进行湿制备,以使用Amsel标准和Whiff检验来鉴定TV和BV诊断。第二个样品用于细菌培养,并使用圆盘扩散技术进行抗菌分析。临床实验室标准研究所(CLSI)解释标准用于对结果进行分类。
结果:客户的平均年龄为28.11±7.08岁。大多数(88%)年龄在15-35岁之间。只有81(34.9%)的微生物被分离或从其标本中看到,其中19(8.2%)的微生物被归类为具有BV(分离的细菌或加德纳菌)。在81名感染者中,33人(40.8%)只有细菌感染,36例(44.4%)仅有TV,12例(14.8%)细菌与TV共感染。从临床记录来看,被分类为患有UTI或阴道炎的人群仅为46人(20.7%)。研究观察到年龄(15-35岁)与阴道病/电视共感染之间的相关性(X2=7.9;P=0.005).有阴道炎或UTI症状的参与者(主要是大肠杆菌和假单胞菌。孤立),BV/TV共感染与女性交易者显着相关(X2=8.5;P=0.003),而与一夫多妻制关系的患者更相关(X2=18.79,P=0.0001)。第三和第二的女人。三个月与阴道感染的相关性更高(X2=9.47,P=0.002;X2=4.79,P=0.029)。假单胞菌对环丙沙星(CIP)和头孢呋辛(CXM)敏感。同时,大肠杆菌分离株对头孢吡肟敏感,环丙沙星,还有亚胺培南.
结论:在所研究的社区中,BV和鞭毛共感染的患病率相对较低。
结论:我们建议对产前有BV和鞭毛共感染症状的妇女进行筛查,以避免其进展为阴道炎。
OBJECTIVE: This study was undertaken to determine the prevalence of Bacterial Vaginosis (BV), Trichomonas Vaginalis (TV) co-infection, and the antibacterial sensitivity profile of bacterial isolates.
METHODS: The study was a cross-sectional study of 232 pregnant women on a routine
antenatal visit between April 2019 and Sept. 2020, at Amukoko clinic in Lagos, Nigeria. The gynaecologist conducted the clinical examination on each patient looking for vaginal discharge and its consistency/homogeneity, colour and odour. Two High Vaginal Swab (HVS) samples were taken from every patient and a semi-structured questionnaire was used to gather the socio-demographic, practices/attitudes, and clinical information of each participant. One sample was employed for wet preparation to identify the TV and BV diagnosis using Amsel\'s criteria and Whiff\'s test. The second sample was used for bacterial culture and antibiogram was conducted using the disc diffusion technique. The Clinical Laboratory Standard Institutes\' (CLSI) interpretative criteria were used to categorise the results.
RESULTS: The mean age of the clients was 28.11 ± 7.08 years of age. The majority (88%) were aged 15-35 years. Only 81 (34.9%) had microbial organisms isolated or seen from their specimens and 19 (8.2%) of such were classified as having BV (Bacteriods or Gardnerella isolated). Of the 81 infected, 33 (40.8%) had only bacterial infection, 36 (44.4%) had TV alone and 12 (14.8%) had bacteria co-infected with TV. From the clinical records, the population that was classified as having UTI or vaginitis was only 46 (20.7%) The study observed age (15-35 years) related association between vaginosis/ TV co-infection (X2 = 7.9; P = 0.005). Participants with symptoms of vaginitis or UTI (mainly E. coli & pseudomonas spp. isolated), BV/co-infection with TV significantly associated with female traders (X2 = 8.5; P = 0.003) and were more associated with those from polygamous relationships (X2 = 18.79, P = 0.0001). Women in their 3rd and 2nd. trimester were more significantly associated with vaginal infection (X2 = 9.47, P = 0.002; X2 = 4.79, P = 0.029) respectively. The Pseudomonas showed susceptibility to ciprofloxacin (CIP) and cefuroxime (CXM). While, E. coli isolates were susceptible to cefepime, ciprofloxacin, and imipenem.
CONCLUSIONS: There is a relatively low prevalence of BV and flagellate co-infection in the community studied.
CONCLUSIONS: We recommend screening of
antenatal women with underlying symptoms for BV and flagellates co-infection to avoid its progression to vaginitis.