The excessive presence of geogenic ammonium (NH4+) in groundwater poses a global environmental concern, commonly linked to the degradation of nitrogen-containing dissolved organic matter (DOM). However, there is a gap in systematic studies on the combination of soluble organic matter (SOM) in sediments and DOM in groundwater, with few indoor incubation experiments to validate their degradation pathways. This study utilized ultrahigh-resolution Fourier transform ion cyclotron resonance mass spectrometry to analyze the molecular characteristics of DOM and SOM in aquifer systems affected by geogenic NH4+. Subsequently, indoor incubation experiments spanning up to 140 d were conducted to verify the degradation pathways. The experimental results revealed a two-phase degradation process for both the DOM and SOM. The initial stage was characterized by the degradation of aliphatic compounds (ALC) with the production of polyphenols (PPE) and highly unsaturated compounds (HUC). The second stage was dominated by the degradation of PPE and HUC, accompanied by the re-consumption of some ALC, while more recalcitrant HUC persisted. Notably, the first stage of SOM degradation exceeded that of DOM degradation, indicating that SOM exhibited greater resistance to aging. This phenomenon may be attributed to a wider range of active enzymes in sediments, the rapid replenishment of SOM by organic matter in sediments, or the accelerated degradation of DOM. The experimental results aligned with the molecular characterization of DOM and SOM in actual aquifer systems. It is hypothesized that NH4+ produced through the direct mineralization of SOM may contribute more to the enrichment of NH4+ in groundwater than that produced through the mineralization of DOM. This study is the first to analyze DOM and SOM together in aquifer systems and validate their degradation pathways through incubation experiments, thereby providing novel insights into the enrichment of geogenic NH4+ in groundwater.

Tire wear particles (TWPs) in stormwater runoff have been widely detected and were generally classified into microplastics (MPs). TWPs and conventional MPs can be intercepted and accumulated in stormwater filtration systems, but their impacts on filtration, adsorption and microbial degradation processes of conventional pollutants (organic matters, nitrate and ammonium) have not been clarified. TWPs are different from MPs in surface feature, chemical components, adsorption ability and leaching of additives, which might lead to their different impacts on conventional pollutants removal. In this study, five different levels of aged polyethylene MPs (PEMPs) and aged TWPs contamination in stormwater filtration systems were simulated using thirty-three filtration columns. Results showed that ultraviolet aging treatment was less influential for the aging of TWPs than that of PEMPs, the specific surface area of aged PEMPs (1.603 m2/g) was over two times of unaged TWPs (0.728 m2/g) in the same size. Aged PEMPs and aged TWPs had different impacts on conventional pollutants removal performance and microbial communities, and the difference might be enlarged with exposure duration. The intensified aged PEMPs contamination generally promoted conventional pollutants removal, whereas aged TWPs showed an opposite trend. Mild contamination (0.01% and 0.1%, wt%) of aged PEMP/TWPs was beneficial to the richness and diversity of microbial communities, whereas higher contamination of aged PEMPs/TWPs was harmful. Aged PEMPs and TWPs had different impact on microbial community structure. Overall, the study found that TWPs were more detrimental than PEMPs in filtration systems. The research underscores the need for more comprehensive investigation into the occurrence, effects and management strategies of TWPs, as well as the importance of distinguishing between TWPs and MPs in future studies.

East African lakes, especially soda lakes, are home habitats for massive numbers of wildlife such as flamingos, mammals, and fishes. These lakes are known for their high primary production due to local high temperatures, light intensities, and alkalinity (inorganic carbon). However, these lakes, normally within remote areas, receive low nutrient inputs. Ammonium (NH4+) recycling and/or nitrogen fixation can become the major N supply mechanisms for phytoplankton. However, the driving forces on microbial N nutrition in lakes with minimal anthropogenic disturbance remain poorly understood. Using stable isotope tracer techniques, NH4+ recycling rates were measured in 18 lakes and reservoirs in East Africa (Tanzania and Kenya) during the dry season in early 2020. Three functional genes (nifH, gdh, and ureC) relating to microbial N nutrition were also measured. The regeneration of NH4+ supported up to 71 % of the NH4+ uptake. Positive community biological NH4+ demands (CBAD) for all lakes and reservoirs indicate an obvious N demand from microbial community. Our study provides clear evidence that microbial NH4+ uptake rates linked closely to the dissolved organic matter (DOM) properties (e.g., the absorption coefficient at 254 nm, percents of total fluorescence intensity contributed by microbial humic-like and protein-like components) and that water residence time drives microbial NH4+ recycling by regulating the duration of in-lake DOM processing and influencing algal growth. Phytoplankton, especially those of Cyanophyceae, showed maximum biomass and higher NH4+ recycling rates at a certain range of water residence time (e.g., 5-8 years). However, CBAD showed a decreasing trend with longer water residence time, which may be influenced by changes in the algal community composition (e.g., % Cyanophyceae vs. % Bacillariophyceae). These results indicate that DOM dynamics and the water residence time have the potential to facilitate the understanding of microbial nitrogen supply status in East African lakes.

BACKGROUND: Previous studies have primarily focused on the links between fine particulate matter (PM2.5) and bone health among the general population instead of PM2.5 components and the specific population. We aimed to investigate the associations between PM2.5 components and bone health among middle-aged and elderly women.
METHODS: A total of 748 middle-aged and elderly women were randomly sampled from 32 streets/villages in Hubei Province. The concentrations of ambient PM2.5 and its components were extracted across various residential areas from the Tracking Air Pollution in China (TAP) dataset. Bone mineral density measurements were obtained from dual-energy X-ray absorptiometry scans of study participants. Multivariable logistic and linear models were employed to assess the relationship between PM2.5 and its components and bone loss.
RESULTS: Per interquartile range (IQR) increase in nitrate (NO3-) and ammonium (NH4+) concentrations were associated with 1.65 [95% confidence intervals (CI): 1.13, 2.30] and 1.77 (95% CI: 1.26, 2.49) times higher odds of osteoporosis, respectively. Long-term exposure to sulfate (SO42-), NO3-, and NH4+ was negatively associated with T-scores and bone mineral density in L1-L4 lumbar vertebrae. In addition, the elderly, women who have experienced menarche after the age of 14, and postmenopausal women were more susceptible to the water-soluble inorganic salts of PM2.5.
CONCLUSIONS: The study emphasizes the need for comprehensive policies addressing air quality, with a specific focus on vulnerable populations such as middle-aged and elderly women. Public health interventions aimed at reducing ambient PM2.5 concentrations and minimizing exposure to its harmful components are crucial in mitigating the adverse impact on skeletal health and improving the overall well-being of communities.

Tropical stream ecosystems are under increasing human pressure, making the development of effective restoration approaches and expanding knowledge in this field urgent. This study evaluated the impact of riparian vegetation restoration and environmental context on stream ecosystem functioning by measuring key ecosystem functions - gross primary production (GPP), ecosystem respiration (ER), and nutrient uptake of ammonium and soluble reactive phosphorus - across ten tropical streams in southeastern Brazil. The streams represented a gradient from clearcut areas (impacted reaches) to relatively pristine conditions (reference reaches), including intermediate stages of vegetation recovery (restored reaches). In the short-term (~15-20 years after restoration), restoration led to reduced GPP akin to reference reaches. Yet, ER did not show the anticipated increase, suggesting a longer timeframe is necessary for restored streams to emulate the functional characteristics of reference reaches. Additionally, the restored reaches did not achieve the nutrient uptake efficiencies observed in both impacted and reference reaches, pointing to a partial recovery of ecosystem function. This study suggests that while riparian vegetation restoration contributes positively to certain aspects of stream function, environmental variables less related to this type of restoration, such as discharge and hydromorphology, significantly influence stream ecosystem functioning, highlighting the importance of considering environmental context in restoration efforts. A more holistic approach, possibly encompassing broader hydromorphological and habitat enhancements, is needed to fully restore ecological processes in these vital ecosystems. These insights are critical for informing future tropical stream restoration projects, advocating the use of ecosystem function metrics as comprehensive indicators of ecological recovery and restoration success.

The ammonia/ammonium (NH3/NH4+, AM) concentration in human erythrocytes (RBCs) is significantly higher than in plasma. Two main possible mechanisms for AM transport, including simple and facilitated diffusion, are described; however, the driving force for AM transport is not yet fully characterized. Since the erythroid ammonium channel RhAG forms a structural unit with anion exchanger 1 (eAE1) within the ankyrin core complex, we hypothesized the involvement of eAE1 in AM transport. To evaluate the functional interaction between eAE1 and RhAG, we used a unique feature of RBCs to swell and lyse in isotonic NH4+ buffer. The kinetics of cell swelling and lysis were analyzed by flow cytometry and an original laser diffraction method, adapted for accurate volume sensing. The eAE1 role was revealed according to (i) the changes in cell swelling and lysis kinetics, and (ii) changes in intracellular pH, triggered by eAE1 inhibition or the modulation of eAE1 main ligand concentrations (Cl- and HCO3-). Additionally, the AM import kinetics was analyzed enzymatically and colorimetrically. In NH4+ buffer, RBCs concentration-dependently swelled and lysed when [NH4+] exceeded 100 mM. Cell swelling and hemolysis were tightly regulated by chloride concentration. The complete substitution of chloride with glutamate prevented NH4+-induced cell swelling and hemolysis, and the restoration of [Cl-] dose-dependently amplified the rates of RBC swelling and lysis and the percentage of hemolyzed cells. Similarly, eAE1 inhibition impeded cell swelling and completely prevented hemolysis. Accordingly, eAE1 inhibition, or a lack of chloride anions in the buffer, significantly decreased NH4+ import. Our data indicate that the eAE1-mediated chloride gradient is required for AM transport. Taken together, our data reveal a new player in AM transport in RBCs.

Forests are the largest carbon sink in terrestrial ecosystems, and the impact of nitrogen (N) deposition on this carbon sink depends on the fate of external N inputs. However, the patterns and driving factors of N retention in different forest compartments remain elusive. In this study, we synthesized 408 observations from global forest 15N tracer experiments to reveal the variation and underlying mechanisms of 15N retention in plants and soils. The results showed that the average total ecosystem 15N retention in global forests was 63.04 ± 1.23%, with the soil pool being the main N sink (45.76 ± 1.29%). Plants absorbed 17.28 ± 0.83% of 15N, with more allocated to leaves (5.83 ± 0.63%) and roots (5.84 ± 0.44%). In subtropical and tropical forests, 15N was mainly absorbed by plants and mineral soils, while the organic soil layer in temperate forests retained more 15N. Additionally, forests retained more N 15 H 4 + $$ {}^{15}\\mathrm{N}{\\mathrm{H}}_4^{+} $$ than N 15 O 3 - $$ {}^{15}\\mathrm{N}{\\mathrm{O}}_3^{-} $$ , primarily due to the stronger capacity of the organic soil layer to retain N 15 H 4 + $$ {}^{15}\\mathrm{N}{\\mathrm{H}}_4^{+} $$ . The mechanisms of 15N retention varied among ecosystem compartments, with total ecosystem 15N retention affected by N deposition. Plant 15N retention was influenced by vegetative and microbial nutrient demands, while soil 15N retention was regulated by climate factors and soil nutrient supply. Overall, this study emphasizes the importance of climate and nutrient supply and demand in regulating forest N retention and provides data to further explore the impacts of N deposition on forest carbon sequestration.

This study explores the enhancement of aqueous zinc-ion batteries (AZIBs) using ammonium-enhanced vanadium oxide cathodes. Density Functional Theory (DFT) calculations reveal that NH4+ incorporation into V6O16 lattices significantly facilitates Zn2+ ion diffusion by reducing electrostatic interactions, acting as a structural lubricant. Subsequent experimental validation using (NH4)2V6O16 cathodes synthesized via a hydrothermal method corroborates the DFT findings, demonstrating remarkable electrochemical stability with a capacity retention of 90% after 2000 cycles at 5 A g-1. These results underscore the potential of NH4+ in improving the performance and longevity of AZIBs, providing a pathway for sustainable energy storage solutions.

The seed germination index (GI) serves as the principal determinant that impedes the integration of aerobic composting products into agricultural lands. The current research work predominantly focuses on exploring the correlation between physical and chemical indicators of the compost products and GI, neglecting the fundamental cause. This study systematically analyzed the composition of GI aqueous extracts from compost products derived from kitchen waste under various composting methodologies, with nitrogen, carbon, and inorganic salt as critical factors. The analytical work concluded that acetic acid, formic acid, and ammonium were the inhibitory factors influencing GI. Validation experiments introduced inhibitory factors, yielding a functional relationship formula depicting GI variations due to a single influential factor. This study conclusively identified acetic acid as the primary constraint, establishing that its inhibitory concentration corresponded to 70 % GI stands at 85 mg/L. This study will provide guidelines for the future research on enhancing aerobic composting techniques.

Solid-state NMR allows for the study of membrane proteins under physiological conditions. Here we describe a method for detection of bound ions in the selectivity filter of ion channels using solid-state NMR. This method employs standard 1H-detected solid-state NMR setup and experiment types, which is enabled by using 15N-labelled ammonium ions to mimic potassium ions.