■子宫内膜异位症是育龄期妇女常见的妇科疾病。关于子宫内膜异位症的发病机理存在许多假设。在土耳其,洋葱(通常称为“洋葱治疗”)的消费是一种流行的治疗方法,用于缓解各种妇科疾病。
■在这项研究中,我们的目的是使用Sprague-Dawley大鼠自体子宫内膜异位症模型评估洋葱鳞茎A.cepa的治疗机制.以前的研究表明,A.cepa具有抗炎作用,抗氧化剂,和抗凋亡特性。我们通过使用苏木精-伊红染色和Ki67免疫组织化学分析来评估子宫内膜异位植入物的病理状况。转化生长因子-β1(TGF-β1)和α-平滑肌肌动蛋白(α-SMA)已被鉴定为促纤维化标志物,相对于在位子宫内膜组织,它们在子宫内膜异位组织中高度过表达。此外,TGF-β1影响子宫内膜异位症的分化和进展。为了量化促纤维化活动,我们使用免疫吸附测定法测量TGF-β1和α-SMA。
■在接受高剂量A.cepa的组中观察到子宫内膜异位植入物的组织学评估评分低于其他组。高剂量A.cepa方案后,Ki67表达降低,其中包括30%的A.cepa和70%的正常饲料。然而,各组间TGF-β1或α-SMA水平无统计学差异(分别为p=0.7和p=0.778).
■研究结果表明,A.cepa可以作为子宫内膜异位症治疗的治疗剂,增殖潜力的降低证明了这一点。然而,A.cepa与子宫内膜异位症相关的TGF-β1或α-SMA的水平显着降低无关。
OBJECTIVE: Endometriosis is a common gynecological disease among reproductive-age women. Numerous hypotheses exist regarding the pathogenesis of endometriosis. In Turkey, the consumption of Allium cepa (commonly known as the \"onion cure\") is a popular treatment employed to alleviate a variety of gynecological disorders.
METHODS: In this study, our objective was to assess the therapeutic mechanisms of the onion bulb A. cepa using an autologous endometriosis model in Sprague-Dawley rats. Previous research has shown that A. cepa possesses anti-inflammatory, antioxidant, and antiapoptotic properties. We evaluated the pathological condition of endometriotic implants by employing hematoxylin-eosin staining and Ki67 immunohistochemistry analysis. Transforming growth factor-beta 1 (TGF-β1) and alpha-smooth muscle actin (α-SMA) have been identified as profibrotic markers that are highly overexpressed in endometriotic tissues relative to eutopic endometrial tissue. Furthermore, TGF-β1 influences the differentiation and progression of endometriosis. To quantify profibrotic activity, we measured TGF-β1 and α-SMA using the immunosorbent assay method.
RESULTS: Lower histologic evaluation scores for endometriotic implants were observed in the group receiving high-dose A. cepa relative to the other groups. Ki67 expression was reduced following the high-dose A. cepa regimen, which consisted of 30% A. cepa and 70% normal feed. However, no statistically significant differences in TGF-β1 or α-SMA levels were observed among the groups (p=0.7 and p=0.778, respectively).
CONCLUSIONS: The findings suggest that A. cepa could serve as a therapeutic agent in endometriosis treatment, as evidenced by the reduction in proliferative potential. Nevertheless, A. cepa was not associated with significantly lower levels of endometriosis-associated TGF-β1 or α-SMA.