背景:已对图拉斯内拉物种的划界进行了广泛研究,主要在形态(性和无性状态)和分子水平-显示它们之间的歧义。一种综合物种概念,包括分子等特征,生态学,形态学,和其他信息对于Tulasnella等复杂群体的物种划界至关重要。
目的:本研究的目的是使用基于对齐和无对齐的距离矩阵的组合作为传统方法的替代分子工具来测试进化关系,并考虑来自ITS2(内部转录间隔区)序列的二级结构和CBC,用于图拉斯内拉的物种定界。
方法:绘制了三种系统发育方法:(i)基于比对,(ii)无对齐,和(iii)使用来自R包的DISATIS和pvclust库的两个距离矩阵的组合。最后,二级结构一致性由Mfold建模,并使用4Sale进行了CBC分析,以补充物种划界。
结论:系统发育树结果显示图拉斯纳氏菌属的单系进化枝。,其中将所有142个图拉斯内拉序列分为两个主要进化枝A和B,并分配给七个物种(T。不对称,T.Andina,T.eichlerianaECU6,T.eichlerianaECU4T.pinicola,T.小提琴),从72%到100%的引导值支持。从2D二级结构对齐,获得了三种具有螺旋和环的共识模型。因此,T.albida属于I型;T.eichleriana,T.流带,T.violea属于II型;T.不对称,T.Andina,T.pinicola,还有T.spp.(GER)属于III型;每个类型包含四到六个域,其中有9个CBCs证实了不同的物种。
BACKGROUND: The delimitation of species of Tulasnella has been extensively studied, mainly at the morphological (sexual and asexual states) and molecular levels-showing ambiguity between them. An integrative species concept that includes characteristics such as molecular, ecology, morphology, and other information is crucial for species delimitation in complex groups such as Tulasnella.
OBJECTIVE: The aim of this study is to test evolutionary relationships using a combination of alignment-based and alignment-free distance matrices as an alternative molecular tool to traditional methods, and to consider the secondary structures and CBCs from ITS2 (internal transcribed spacer) sequences for species delimitation in Tulasnella.
METHODS: Three phylogenetic approaches were plotted: (i) alignment-based, (ii) alignment-free, and (iii) a combination of both distance matrices using the DISTATIS and pvclust libraries from an R package. Finally, the secondary structure consensus was modeled by Mfold, and a CBC analysis was obtained to complement the species delimitation using 4Sale.
CONCLUSIONS: The phylogenetic tree results showed delimited monophyletic clades in Tulasnella spp., where all 142 Tulasnella sequences were divided into two main clades A and B and assigned to seven species (T. asymmetrica, T. andina, T. eichleriana ECU6, T. eichleriana ECU4 T. pinicola, T. violea), supported by bootstrap values from 72% to 100%. From the 2D secondary structure alignment, three types of consensus models with helices and loops were obtained. Thus, T. albida belongs to type I; T. eichleriana, T. tomaculum, and T. violea belong to type II; and T. asymmetrica, T. andina, T. pinicola, and T. spp. (GER) belong to type III; each type contains four to six domains, with nine CBCs among these that corroborate different species.