猕猴桃(猕猴桃属。)容易受到淹水压力。尽管猕猴桃植物中存在丰富的野生种质资源,以提高猕猴桃品种的耐涝能力。潜在的机制在很大程度上仍然未知。这里,使用一种野生种质进行了比较研究,毛仁神(A.ValvataDunn,MRS),和一个品种,米良-1(A.中国.deliciosa(A.切夫)A.雪佛兰。cv.米良-1,ML)。在压力下,ML植株严重受损,枯萎的落叶和变黑的腐烂根,而MRS植株的损伤症状要少得多,随着更高的光合速率,胁迫条件下的叶绿素荧光特性和根系活性。然而,胁迫暴露后,两种种质均未出现根中的常理组织或不定根。丙酮酸脱羧酶(PDC)和乙醇脱氢酶(ADH)的活性,以及他们的成绩单水平,在正常和应激条件下,MRS的组成性均高于ML。淹水胁迫显著增强两种种质的PDC和ADH酶活性,在淹水胁迫下,MRS根比ML根高60.8%和22.4%,分别。此外,MRS显示出较高的抗氧化酶活性,包括SOD,CAT,APX,以及DPPH-自由基清除能力,在正常和胁迫条件下,H2O2和MDA的积累均减少。我们的发现表明,野生有价曲霉种质的耐涝性与高PDC和ADH有关,以及抗氧化能力。
Kiwifruit (Actinidia spp.) is susceptible to waterlogging stress. Although abundant wild germplasm resources exist among Actinidia plants for improving the waterlogging tolerance of kiwifruit cultivars, the underlying mechanisms remain largely unknown. Here, a comparative study was undertaken using one wild germplasm, Maorenshen (A. valvata Dunn, MRS), and one cultivar, Miliang-1 (A. chinensis var. deliciosa (A.Chev.) A.Chev. cv. Miliang-1, ML). Under stress, the ML plantlets were seriously damaged with wilted chlorotic leaves and blackened rotten roots, whereas the symptoms of injury in the MRS plantlets were much fewer, along with higher photosynthetic rates, chlorophyll fluorescence characteristics and root activity under stress conditions. However, neither
aerenchyma in the root nor adventitious roots appeared in both germplasms upon stress exposure. The activities of pyruvate decarboxylase (PDC) and alcohol dehydrogenase (ADH), as well as their transcript levels, were constitutively higher in MRS than those in ML under both normal and stress conditions. Waterlogging stress significantly enhanced the PDC and ADH enzyme activities in both germplasms, which were 60.8% and 22.4% higher in the MRS roots than those in the ML roots under waterlogging stress, respectively. Moreover, MRS displayed higher activities of antioxidant enzymes, including SOD, CAT, and APX, as well as DPPH-radical scavenging ability, and decreased H2O2 and MDA accumulation under both normal and stress conditions. Our findings suggest that the waterlogging tolerance of the wild A. valvata germplasm was associated with high PDC and ADH, as well as antioxidant ability.