鉴定参与应激源反应的关键基因和分子途径对于控制鱼类和可持续水产养殖的应激至关重要。环境应激源可以诱导水生动物的应激反应,导致免疫功能受损,抑制生长,死亡率上升。mRNA-seq分析提供了鉴定与应激反应相关的关键基因和途径的有力工具。在本研究中,mRNA-seq分析用于确定盐度下的关键重叠差异表达基因(DEG)和分子途径。亚硝酸盐,铜,尼罗罗非鱼(Oreochromisniloticus)肝脏中的pH值胁迫。与免疫反应相关的途径,氧气输送,稳态,氧化应激在所有压力源中都富集。最上面的KEGG途径是补体和凝血级联,PPAR信号通路,和心肌收缩。最高的GO富集术语是氧化还原酶活性,有氧呼吸,内肽酶抑制剂活性,内肽酶调节活性,血红素结合,和铁离子结合。补体基因(C3,C4,C5,因子B,和系数H),α-2-巨球蛋白(A2M),血红蛋白亚基ε(HBE),血红蛋白亚基α(HBA),凝血因子基因(XI和X)和细胞色素c氧化酶(COX)基因家族(cox1,cox2,cox3,细胞色素P450)被鉴定为跨多个应激源的关键共享基因。这些基因和分子途径的发现为尼罗罗非鱼应激反应的分子机制提供了更好的理解。本研究的结果可以促进尼罗罗非鱼压力管理策略的发展。
The identification of key genes and molecular pathways that are involved in the response to stressors is crucial for controlling stress in fish and sustainable aquaculture. Environmental stressors can induce stress responses in aquatic animals, resulting in compromised immune function, inhibited growth, and increased mortality rates. mRNA-seq analysis provides a powerful tool to identify key genes and pathways associated with stress response. In the present study, mRNA-seq analysis was employed to identify key overlapping differentially expressed genes (DEGs) and molecular pathways under salinity, nitrite, copper, and pH stress in the liver of Nile tilapia (Oreochromis niloticus). The pathways associated with the immune response, oxygen transport, homeostasis, and oxidative stress were enriched across all stressors. The top KEGG pathways were complement and coagulation cascades, PPAR signaling pathway, and cardiac muscle contraction. The top GO enrichment terms were oxidoreductase activity, aerobic respiration, endopeptidase inhibitor activity, endopeptidase regulator activity, heme binding, and iron ion binding. The complement genes (C3, C4, C5, factor B, and factor H), alpha-2-macroglobulin (A2M), hemoglobin subunit epsilon (HBE), hemoglobin subunit alpha (HBA), coagulation factor genes (XI and X) and the cytochrome c oxidase (COX) gene family (cox1, cox2, cox3, cytochrome P450) were identified as key shared genes across multiple stressors. The discovery of these genes and molecular pathways provided a better understanding of the molecular mechanism underlying the stress response in Nile tilapia. The results of the present study can facilitate the development of stress management strategies in Nile tilapia.