■瘤胃酸中毒是肉牛最常见的疾病之一。严重影响小牛的正常发育,对养殖业构成重大威胁。然而,瘤胃酸中毒对小牛肠道菌群和血清代谢产物的影响目前尚不清楚。
■本研究的目的是研究瘤胃酸中毒后小牛肠道菌群和血清代谢产物的变化并分析其相关性。
■选择8只小牛作为瘤胃酸中毒组,选取健康小牛8只作为健康组。采用16SrDNA高通量测序和非靶代谢组学方法分别检测小牛的粪便肠道菌群和血清代谢产物。采用Spearman相关分析法分析肠道菌群与血清代谢产物的相关性。
■对8只雄性健康(Health)和8只雄性瘤胃酸中毒(Disease)小牛的肠道菌群多样性和组成的差异分析显示,瘤胃酸中毒增加了小牛肠道菌群的丰度。在门一级,与健康组相比,疾病组的变形杆菌相对丰度显著降低(P<0.05),而疾病组脱硫杆菌的相对丰度显著增加(P<0.05)。在属一级,与疾病组相比,Alloprevotella的相对丰度,Muribaculaceae,丁氏弧菌,普雷沃氏菌,健康组杆菌属和副杆菌属明显升高(P<0.05),而健康组Christensellaceae_R-7和单球形的相对丰度显着降低(P<0.05)。差异分析结果显示健康组有23属丰度较高,而疾病组有47属,丰度较高。血清代谢组学结果揭示了与瘤胃酸中毒相关的差异代谢产物,包括烟酰胺,烟酸,L-谷氨酸和肌肽,主要富集在烟酸、烟酰胺途径和组氨酸途径。
■瘤胃酸中毒的发生可引起小牛肠道菌群的变化,随着Christensenellaceae_R-7属的显着增加,而Prevotella和白弧菌属的显着减少。此外,瘤胃酸中毒的发生也可引起血清代谢产物包括烟酸的变化,烟酰胺,L-谷氨酰胺,和肌肽,这可能是诊断小牛瘤胃酸中毒的生物标志物。
UNASSIGNED: Rumen
acidosis is one of the most common diseases in beef cattle. It severely affects the normal development of calves and poses a significant threat to the farming industry. However, the influence of rumen
acidosis on the gut microbiota and serum metabolites of calves is currently unclear.
UNASSIGNED: The aim of this study is to investigate the changes in the gut microbiota and serum metabolites in calves after rumen acidosis and analyse the correlation.
UNASSIGNED: Eight calves were selected as the rumen
acidosis group, and eight health calves were selected as the healthy group. The faecal gut microbiota and serum metabolites of calves were detected respectively using 16S rDNA high-throughput sequencing and non-target metabolomics. The correlation between gut microbiota and serum metabolites was analyzed by Spearman correlation analysis.
UNASSIGNED: Differential analysis of the diversity and composition of gut microbiota between eight male healthy (Health) and eight male rumen acidosis (Disease) calves revealed that rumen
acidosis increased the abundance of the gut microbiota in calves. At the phylum level, compared to the Healthy group, the relative abundance of Proteobacteria in the Disease group significantly decreased (P<0.05), while the relative abundance of Desulfobacterota significantly increased in the Disease group (P<0.05). At the genus level, compared to the Disease group, the relative abundance of Alloprevotella, Muribaculaceae, Succinivibrio, Prevotella, Agathobacter and Parabacteroides significantly increased in the Healthy group (P<0.05), while the relative abundance of Christensenellaceae_R-7 and Monoglobus significantly decreased in the Healthy group (P<0.05). Differential analysis results showed the Healthy group had 23 genera with higher abundance, while the Disease group had 47 genera with higher abundance. Serum metabolomics results revealed the differential metabolites associated with rumen acidosis, including nicotinamide, niacin, L-glutamic acid and carnosine, were mainly enriched in the nicotinate and nicotinamide pathway and the histidine pathway.
UNASSIGNED: The occurrence of rumen acidosis can induce changes in the gut microbiota of calves, with a significant increase of the Christensenellaceae_R-7 genus and a significant decrease of Prevotella and Succinivibrio genera. In addition, the occurrence of rumen
acidosis can also induce changes in serum metabolites including niacin, niacinamide, L-glutamine, and carnosine, which may serve as the diagnostic biomarkers of rumen acidosis of calves.