神经活性物质或药理学试剂的位点特异性施用是神经学研究中的常规实践。为了促进慢性治疗,通常将颅内插管植入颅骨中。这些外科手术被广泛描述和记录为啮齿动物,作为最常见的动物模型,甚至经过多年的精炼。然而,对幼龄肉鸡第三脑室插管的正确程序的精确描述尚不清楚。这种缺席令人惊讶,因为鸡模型通常用于研究代谢和摄取的生理控制。此外,作为一种商业动物,在阐明饲料摄入和代谢的中心调节以优化肉鸡的饲养和生活条件方面也有特别的兴趣。作为摄食和代谢调节中心的脑核是弓形核,下丘脑腹内侧核,和下丘脑外侧,它们都位于第三脑室附近。这里,我们为7天大的肉鸡提供脑室内(i.c.v.)插管的完整方案,以及对啮齿动物i.c.v.插管取得的一些进展的审查,以及这些进展是否适用于鸡的插管。使用这里描述的外科手术,我们能够实现88%的正确植入成功率,死亡率低于1%(n=224)。•在7d龄肉鸡的第三脑室内植入管的详细程序。•在肉鸡中需要带有锚固螺钉的水泥帽,胶水不能提供足够的稳定性。•测试羧酸盐水泥和自粘树脂水泥作为甲基丙烯酸甲酯水泥的替代物。
The site-specific administration of neuroactive substances or pharmacological agents is a routine practice in neurological studies. To facilitate chronic treatments intercranial cannulae are often implanted in the skull. These surgical procedures are widely described and well-documented for rodents, as the most common animal model, and even refined over the years. However, precise descriptions of proper procedure for third ventricle cannulation in young broiler chicks is not readily available. This absence is surprising, as chicken models are often used in the investigation of physiological control of the metabolism and ingestion. Furthermore, as a commercial animal, there is also a particular interest in elucidating the central regulation of feed intake and metabolism to optimize feeding and living conditions for broilers. The brain nuclei that serve as the regulatory centers of feeding and metabolism are the arcuate nucleus, the ventromedial hypothalamic nucleus, and the lateral hypothalamus, which are all located near the third ventricle. Here, we provide a full protocol for intracerebroventricular (i.c.v.) cannulation of 7-day old broiler chicks, as well as a review of some of the advances made in rodent i.c.v. cannulations and whether these advances are applicable to cannulations in chickens. Using the surgical procedure described here, we were able to achieve a success rate of proper implantation of 88%, with a mortality rate of less than 1% (n = 224).•Detailed procedure for i.c.v. canula implantation in the third ventricle of 7d-old broiler chicks.•Cement cap with anchoring screws is necessary in broilers, glue does not provide sufficient stability.•Carboxylate cement and self-adhesive resin cement were tested as an alternative for methyl methacrylate cement.