■鳗草是一种典型的海洋被子植物,对高盐环境表现出很强的适应性。先前的研究表明,在鳗草中硝酸还原酶(NR)合成一氧化氮(NO)的途径被阻断后,各种生长和生理指标受到显着影响。
■分析NO对鳗草高盐环境适应性的分子机理,我们用人工海水(对照组)和1mM/LNa2WO4人工海水(实验组)处理鳗草。基于转录组学和代谢组学,探讨了NO影响鳗草耐盐性的分子机制。
■我们通过转录组测序在鳗草根中获得了326、368和859个差异表达基因(DEGs),茎,和叶子,分别。同时,我们通过代谢组学在根中获得了63、52和36种差异积累的代谢物(DAMs),茎,和叶子,分别。最后,通过转录组和代谢组的联合分析,我们发现鳗草根和叶的NO调控机制与陆生植物相似,而茎的调控机制具有相似和独特的特点。
■鳗草根中的NO通过影响跨膜转运和茉莉酸相关途径中的基因来调节渗透平衡和抗氧化防御,以提高鳗草对高盐环境的适应性。鳗草叶片中的NO通过影响植物激素的信号转导来调节下游抗氧化防御系统。鳗草茎中的NO通过影响与离子稳态相关的基因来调节离子稳态,以增强鳗草对高盐环境的适应性。不同的是,在NO合成受到抑制后,乙醛酸和二羧酸代谢,以及三羧酸(TCA)循环,受葡萄糖代谢调节,作为应对鳗草茎中高盐环境的补充作用。这些是对鳗草中NO调节机制的研究,为海洋植物耐盐机理的研究和陆生作物性状的改良提供理论依据。本研究发现的关键基因可通过克隆和分子育种方法应用于提高陆生作物的耐盐性。
UNASSIGNED: Eelgrass is a typical marine angiosperm that exhibits strong adaptability to high-salt environments. Previous studies have shown that various growth and physiological indicators were significantly affected after the nitrate reductase (NR) pathway for nitric oxide (NO) synthesis in eelgrass was blocked.
UNASSIGNED: To analyze the molecular mechanism of NO on the adaptability to high-salt environment in eelgrass, we treated eelgrass with artificial seawater (control group) and artificial seawater with 1 mM/L Na2WO4 (experimental group). Based on transcriptomics and metabolomics, we explored the molecular mechanism of NO affecting the salt tolerance of eelgrass.
UNASSIGNED: We obtained 326, 368, and 859 differentially expressed genes (DEGs) by transcriptome sequencing in eelgrass roots, stems, and leaves, respectively. Meanwhile, we obtained 63, 52, and 36 differentially accumulated metabolites (DAMs) by metabolomics in roots, stems, and leaves, respectively. Finally, through the combined analysis of transcriptome and metabolome, we found that the NO regulatory mechanism of roots and leaves of eelgrass is similar to that of terrestrial plants, while the regulatory mechanism of stems has similar and unique features.
UNASSIGNED: NO in eelgrass roots regulates osmotic balance and antioxidant defense by affecting genes in transmembrane transport and jasmonic acid-related pathways to improve the adaptability of eelgrass to high-salt environments. NO in eelgrass leaves regulates the downstream antioxidant defense system by affecting the signal transduction of plant hormones. NO in the stems of eelgrass regulates ion homeostasis by affecting genes related to ion homeostasis to enhance the adaptability of eelgrass to high-salt environments. Differently, after the NO synthesis was inhibited, the glyoxylate and dicarboxylate metabolism, as well as the tricarboxylic acid (TCA) cycle, was regulated by glucose metabolism as a complementary effect to cope with the high-salt environment in the stems of eelgrass. These are studies on the regulatory mechanism of NO in eelgrass, providing a theoretical basis for the study of the salt tolerance mechanism of marine plants and the improvement of terrestrial crop traits. The key genes discovered in this study can be applied to increase salt tolerance in terrestrial crops through cloning and molecular breeding methods in the future.