在河马途径中,河马和疣(Wts)激酶的激活导致Yorkie(Yki)的磷酸化,以防止其核移位。虾养殖受到弧菌属细菌的威胁。在这项研究中,我们研究了Hippo途径在太平洋白虾对虾对副溶血性弧菌免疫防御中的作用。我们显示副溶血性弧菌感染促进Yki的表达并促进Yki的去磷酸化和核易位,表明细菌感染时Hippo信号的抑制。Hippo通路成分之间存在复杂的调控关系,Wts,Yki和免疫相关转录因子Dorsal,Relish,和STAT。Hippo和Wts的沉默减弱了血细胞的吞噬作用,Yki的沉默增强了它,提示河马信号激活对虾细胞免疫的正向调节。Hippo和Wts的体内沉默降低了副溶血性弧菌感染的虾的存活率,并提高了组织中的细菌含量,而Yki的沉默显示出相反的结果。这表明Hippo信号的激活和Yki的抑制增强了虾的抗菌免疫。
In the Hippo pathway, activation of Hippo and Warts (Wts) kinases results in the phosphorylation of Yorkie (
Yki), to prevent its nuclear translocation. Shrimp aquaculture is threatened by Vibrio genus bacteria. In this study, we examine the role of the Hippo pathway in immune defense against Vibrio parahaemolyticus in Pacific white shrimp Penaeus vannamei. We show that V. parahaemolyticus infection promotes the expression of Yki and facilitates the dephosphorylation and nuclear translocation of
Yki, indicating the inhibition of Hippo signaling upon bacterial infection. There is a complex regulatory relationship between the Hippo pathway components Hippo, Wts, and
Yki and the immune-related transcription factors Dorsal, Relish, and STAT. Silencing of Hippo and Wts weakened hemocyte phagocytosis, while the silencing of Yki enhanced it, suggesting a positive regulation of shrimp cellular immunity by Hippo signaling activation. In vivo silencing of Hippo and Wts decreased the survival rates of V. parahaemolyticus-infected shrimp and elevated the bacterial content in tissues, while the silencing of
Yki showed the opposite results. This suggests that the activation of Hippo signaling and the inhibition of
Yki enhance antibacterial immunity in shrimp.