进行这项研究是为了评估在简单和复杂的饮食中单独添加β-甘露聚糖酶或与多糖酶复合物组合是否可以改善饮食消化率,营养和能量代谢,断奶猪的肠道健康。将28天断奶的30头猪(7.9公斤±0.851公斤)随机分成2×3阶乘排列,考虑简单(以玉米和豆粕为基础的饮食)或复杂饮食(豆粕含量减少13%,5%的乳清功率,与简单饮食相比,喷雾干燥血浆为2.5%)和不添加任何饮食(对照)或添加β-甘露聚糖酶(BM;0.300g/kg饮食)或β-甘露聚糖酶加上多糖酶复合混合物,例如木聚糖酶,β-葡聚糖酶,和阿拉伯呋喃糖苷酶(BMMCC;0.3000.050g/kg饮食)断奶后17天。在第11-17天收集总粪便和尿液样品。从所有猪收集粪便样品以使用商业ELISA测试鉴定粪便生物标志物。在实验期结束时从所有猪收集血液样品以评估急性期蛋白的血清浓度。在第18天对所有猪实施安乐死以收集肠组织。与复杂饮食相比,简单饮食具有更大的蛋白质消化率和代谢系数(p<0.05)。与对照饮食相比,在BM和BM+MCC中观察到更大(p<0.05)的能量消化率和能量代谢系数。平均而言,BM提高了64kcal/kg,BMMCC提高了100kcal/kg的代谢能。此外,饮食中添加BM和BM+MCC导致较低的粪便水分和粪便输出。此外,BM和BM+MCC饮食也将粪便钙卫蛋白浓度降低了29%和46%,分别,与对照猪相比(p<0.001)。我们得出的结论是,简单的饮食是复杂饮食的合适替代品,不影响断奶后猪的营养消化率和肠道健康。外源酶的添加提高了营养和能量的利用率,以及吸收面积,并降低钙卫蛋白浓度。
This study was conducted to evaluate whether adding β-mannanase alone or in combination with a multi-carbohydrase complex to simple and complex diets could improve diet digestibility, nutrient and energy metabolism, and gut health in weaned pigs. Thirty pigs (7.9 kg ± 0.851 kg) weaned at 28 days were randomly split into a 2 × 3 factorial arrangement, considering a simple (corn and soybean meal-based diet) or complex diet (13% point reduction in inclusion of soybean meal, 5% of whey power, and 2.5% of spray-dried plasma compared to the simple diet) and diet without any addition (control) or the addition of β-mannanase (BM; 0.300 g/kg of the diet) or β-mannanase plus a multi-carbohydrase complex blend such as xylanase, β-glucanase, and arabinofuranosidases (BM + MCC; 0.300 + 0.050 g/kg of the diet) for 17 days post-weaned. Total fecal and urine samples were collected on days 11-17. Fecal samples were collected from all pigs to identify fecal biomarkers using commercial ELISA tests. Blood samples were collected from all pigs at the end of the experimental period to assess serum concentrations of acute-phase proteins. All pigs were euthanized on day 18 for intestinal tissue collection. The simple diet had greater (p < 0.05) protein digestibility and metabolizability coefficients than the complex diet. Greater (p < 0.05) energy digestibility and energy metabolizability coefficients were observed in the BM and BM+ MCC compared to the control diet. On average, BM improved by 64 kcal/kg and BM + MCC improved by 100 kcal/kg of metabolizable energy. Furthermore, the addition of BM and BM + MCC to the diets led to lower fecal moisture and fecal output. Moreover, the BM and BM + MCC diets also reduced fecal calprotectin concentrations by 29 and 46%, respectively, compared to control pigs (p < 0.001). We conclude that simple diets are a suitable alternative to complex diets, without compromising the nutrient digestibility and gut health of post-weaned pigs. The addition of exogenous enzymes improves nutrient and energy utilization, as well as the absorption area, and decreases calprotectin concentrations.