背景:呼吸系统疾病和哮喘,特别是,现在是一个全球性的健康问题。在卢旺达,一些传统的治疗者声称可以用植物性的食谱来治疗哮喘,到目前为止还没有科学证据.
目的:我们的研究旨在评估卢旺达使用的植物配方对哮喘的毒性和抗炎作用,以选择潜在的候选物用于进一步表征活性化合物。
方法:使用CellTiter-Glo发光细胞活力测定,将来自选定民俗配方的水(水性)和甲醇-二氯甲烷(有机)提取物提交给THP-1衍生的巨噬细胞进行毒性测试。使用对THP-1衍生的巨噬细胞的Caspase-Glo1炎性体测定进行植物提取物的抗炎作用的评估。
结果:大多数有机和水性提取物均显示超过95%的细胞活力高达200µg/ml,除了R03Cn有机提取物抑制25%的细胞活力。植物提取物以剂量依赖性方式抑制THP-1衍生的巨噬细胞中的caspase-1活化。四种提取物(R03Cn和R07Kn水提取物,R10MK和R19Sz有机提取物)强烈下调了caspase-1的激活(在50µg/ml时超过70%)。总的来说,有机提取物比水性提取物表现出更好的caspase-1抑制作用。
结论:抑制炎症小体/caspase-1是哮喘的关键作用机制之一。一些传统配方对这种机制具有活性,因此是治疗哮喘和其他炎性体介导的疾病的有力候选者。需要进一步的研究来表征活性分子。
BACKGROUND: Respiratory diseases and asthma, in particular, are nowadays a global health problem. In Rwanda, some traditional healers claim to treat asthma with plant-based recipes, though there is no scientific proof so far.
OBJECTIVE: Our study aimed at evaluating the toxicity and the anti-inflammatory effect of plant recipes used in Rwanda against asthma in order to select potential candidates for further characterization of the active compounds.
METHODS: Water (aqueous) and methanol-dichloromethane (organic) extracts from selected folkloric recipes were submitted for toxicity test on THP-1 derived macrophages using CellTiter-Glo Luminescent Cell Viability Assay. The evaluation of the anti-inflammatory effect of the plant extracts was carried out using the Caspase-Glo 1 Inflammasome assay on THP-1 -derived macrophages.
RESULTS: Most of both organic and aqueous extract showed more than 95% of cell viability up to 200 µg/ml, except for R03Cn organic extract that inhibited 25% of the cell viability. Plant extracts inhibited caspase-1 activation in THP-1 derived macrophages in a dose-dependent manner. Four extracts (R03Cn and R07Kn aqueous extracts, R10MK and R19Sz organic extracts) strongly downregulated the activation of caspase-1 (more than 70% at 50 µg/ml). In general, organic extracts exhibited better caspase-1 inhibitory effects than their aqueous counterparts.
CONCLUSIONS: The inhibition of inflammasome/caspase-1 is one of key mechanisms of action in asthma. Some traditional recipes are active on this mechanism and are thus strong candidates for the treatment of asthma and other inflammasome-mediated diseases. Further investigations are needed to characterize active molecules.