粗乙醇提取物介导的氧化铜纳米颗粒(CuO)的绿色合成和阿铁线虫的正丁醇部分代表了纳米技术的开创性方法,将生态可持续性与先进功能相结合。这种创新的方法利用了存在于A.venustum中的天然生物活性化合物来生产CuO纳米颗粒,表现出显著的抗氧化剂,抗炎,抗菌,和抗增殖特性。使用各种技术对绿色合成的纳米粒子进行了表征,由于XRD证实了CuO纳米粒子的结晶性质,CuO-C的微晶尺寸为14.65nm,CuO-B的微晶尺寸为18.73nm。使用透射电子显微镜显微照片确定CuO-C(14.09±0.17nm)和CuO-B(67.88±2.08nm)的晶粒尺寸。此外,合成的纳米材料和粗乙醇提取物,正丁醇馏分,检查了它们的生物潜力,即抗氧化剂,抗炎,抗菌,和抗HeLa癌细胞的抗增殖活性。在合成的纳米材料中,通过利用正丁醇部分合成的氧化铜纳米颗粒已出现作为潜在的生物医学试剂。CuO-B作为抗氧化剂出现,IC50值为44.63±0.49µg/mL,48.49±0.17µg/mL,DPPH为35.39±0.61µg/mL,FRAP,减少功率测定,分别。此外,CuO-B对革兰氏阳性的显着抗菌潜力(S.金黄色葡萄球菌MIC46.88µg/mL)和革兰氏阴性(K。肺炎MIC23.48µg/mL)细菌菌株也不能忽视。伴随着这个,CuO-B对HeLa细胞的IC50值(138.07µg/mL)证明它是一种潜在的抗癌药物。因此,这种新颖的方法强调了这些合成的纳米粒子具有巨大的生物潜力,可以应用于农业和生物医学的各个领域。
The green synthesis of copper oxide nanoparticles (CuO) mediated by crude ethanolic extract and the n-butanol fraction of Adiantum venustum represents a groundbreaking approach in nanotechnology, combining ecological sustainability with advanced functionality. This innovative method leverages the natural bioactive compounds present in A. venustum to produce CuO nanoparticles, which exhibit remarkable antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative properties. The green synthesized nanoparticles were characterized using a variety of techniques, as XRD confirmed the crystalline nature of the CuO nanoparticles, with a crystallite size of 14.65 nm for CuO-C and 18.73 nm for CuO-B. The grain sizes of CuO-C (14.09 ± 0.17 nm) and CuO-B (67.88 ± 2.08 nm) were determined using transmission electron microscopy micrographs. Furthermore, the synthesized nanomaterial and the crude ethanolic extract, n-butanol fraction, were examined for their biological potentials namely antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative activity against HeLa cancer cells. Among the synthesized nanomaterials, copper oxide nanoparticles synthesized by utilizing the n-butanol fraction have appeared as a potential biomedical agent. CuO-B has arisen as an antioxidant agent with IC50 values of 44.63 ± 0.49 µg/mL, 48.49 ± 0.17 µg/mL, and 35.39 ± 0.61 µg/mL for DPPH, FRAP, and reducing power assay, respectively. Furthermore, the significant antibacterial potential of CuO-B against gram-positive (S. aureus MIC 46.88 µg/mL) and gram-negative (K. pneumonia MIC 23.48 µg/mL) bacterial strains cannot be neglected either. Along with this, the IC50 value (138.07 µg/mL) of CuO-B against HeLa cells proved it to be a potential anticancerous agent. Hence, this novel approach emphasized that these synthesized nanoparticles have tremendous biological potential and can be applied to various fields of agriculture and biomedicine.