由于肌肉色素沉着减少而造成的经济损失突出了黑色素为基础的着色在乌骨鸡肉中的关键作用。就分子机制而言,目前对乌骨鸡胸肌中的黑色素发生了解甚少。这项研究采用全转录组测序来分析黑骨鸡的黑白胸肌样本,导致367个差异表达(DE)mRNA的鉴定,48DElncRNAs,104个DEcircRNAs,和112个参与黑色素沉积的DEmiRNA。基于这些发现,为了更好地理解黑色素沉积的复杂机制,开发了竞争性内源性RNA(ceRNA)网络.此外,我们的分析揭示了关键的DMRNAs(TYR,DCT,EDNRB,MLPH和OCA2)受DEmiRNA(gga-miR-140-5p,gga-miR-1682,gga-miR-3529,gga-miR-499-3p,novel-m0012-3p,gga-miR-200b-5p,gga-miR-203a,gga-miR-6651-5p,gga-miR-7455-3p,gga-miR-31-5p,miR-140-x,miR-455-x,novel-m0065-3p,gga-miR-29b-1-5p,miR-455-y,novel-m0085-3p,和gga-miR-196-1-3p)。这些DEmiRNAs与DElncRNAs竞争性相互作用,包括MSTRG.2609.2、MSTRG.4185.1、LOC112530666、LOC112533366、LOC771030、LOC107054724、LOC121107411、LOC100859072、LOC101750037、LOC121108550、LOC121109224、LO如novel_circ_000158、novel_circ_000623、novel_001518和novel_circ_003596。这项研究的发现提供了对调节lncRNA的机制的见解,circRNA,miRNA,和mRNA在鸡黑色素沉积中的表达。
The economic losses incurred due to reduced muscle pigmentation highlight the crucial role of melanin-based coloration in the meat of black-bone chickens. Melanogenesis in the breast muscle of black-bone chickens is currently poorly understood in terms of molecular mechanisms. This study employed whole-transcriptome sequencing to analyze black and white breast muscle samples from black-bone chickens, leading to the identification of 367 differentially expressed (DE) mRNAs, 48 DElncRNAs, 104 DEcircRNAs, and 112 DEmiRNAs involved in melanin deposition. Based on these findings, a competitive endogenous RNA (ceRNA) network was developed to better understand the complex mechanisms of melanin deposition. Furthermore, our analysis revealed key DEmRNAs (TYR, DCT, EDNRB, MLPH and OCA2) regulated by DEmiRNAs (gga-miR-140-5p, gga-miR-1682, gga-miR-3529, gga-miR-499-3p, novel-m0012-3p, gga-miR-200b-5p, gga-miR-203a, gga-miR-6651-5p, gga-miR-7455-3p, gga-miR-31-5p, miR-140-x, miR-455-x, novel-m0065-3p, gga-miR-29b-1-5p, miR-455-y, novel-m0085-3p, and gga-miR-196-1-3p). These DEmiRNAs competitively interacted with DElncRNAs including MSTRG.2609.2, MSTRG.4185.1, LOC112530666, LOC112533366, LOC771030, LOC107054724, LOC121107411, LOC100859072, LOC101750037, LOC121108550, LOC121109224, LOC121110876, and LOC101749016, as well as DEcircRNAs, such as novel_circ_000158, novel_circ_000623, novel_001518, and novel_circ_003596. The findings from this study provide insight into the mechanisms that regulate lncRNA, circRNA, miRNA, and mRNA expression in chicken melanin deposition.