商业藻类藻酸盐的结构不一致限制了它们在各种应用中的可靠性和质量。因此,结构一致的藻酸盐的生物合成对于替代藻酸盐是至关重要的。因此,本研究旨在探讨铜绿假单胞菌CMG1418的结构和功能特性。为了实现这一点,CMG1418藻酸盐使用各种技术如透射电子显微镜进行了物理化学表征,傅里叶变换红外1H-NMR,13C-NMR,和凝胶渗透色谱法。然后对合成的CMG1418藻酸盐进行标准测试以评估其生物相容性,乳化,亲水性,絮凝,胶凝,和流变特性。分析研究表明,CMG1418藻酸盐是一种细胞外的多分散聚合物,分子量范围为20000-250000Da。它包含76%的聚-(1-4)-β-D-甘露糖醛酸(M-嵌段),没有聚-α-L-古洛糖醛酸(G-嵌段),12%β-D-甘露糖醛酸和α-L-古洛糖醛酸的交替序列(聚-MG/GM-嵌段),12%MGM块,172度的聚合度,和M-残基的二-O-乙酰化。有趣的是,CMG1418藻酸盐不显示任何细胞毒性或抗代谢活性。此外,与藻类藻酸盐相比,CMG1418藻酸盐在广泛的pH和温度范围内表现出更高且更稳定的絮凝效率(70-90%)和粘度(4500-4760cP)。此外,它显示出软到柔韧的胶凝能力和更高的持水能力(375%)。它还显示了热力学上更稳定的乳化活性(99-100%),超过了藻类藻酸盐和市售乳化剂。然而,只有二价和多价阳离子可以稍微增加粘度,胶凝,和絮凝。总之,这项研究探索了结构二O-乙酰化和聚G-嵌段缺陷,生物相容性藻酸盐,及其pH值和热稳定功能特性。这项研究表明,在各种应用中,CMG1418藻酸盐是藻类藻酸盐的优越且更可靠的替代品,例如增粘,软胶凝,絮凝,乳化,和水持有。
The structural inconsistencies in commercial algal alginates have limited their reliability and quality for various applications. Therefore, the biosynthesis of structurally consistent alginates is crucial to replace the algal alginates. Thus, this study aimed to investigate the structural and alginate\'s structural and functional properties of Pseudomonas aeruginosa CMG1418 as a substitute. To achieve this, the CMG1418 alginates were physiochemically characterized using various techniques such as transmission electron microscopy, Fourier-transform infrared 1H-NMR, 13C-NMR, and gel permeation chromatography. The synthesized CMG1418 alginate was then subjected to standard tests to evaluate its biocompatibility, emulsification, hydrophilic, flocculation, gelling, and rheological properties. The analytical studies revealed that CMG1418 alginate is an extracellular and polydisperse polymer with a molecular weight range of 20 000-250 000 Da. It comprises 76% poly-(1-4)-β-D-mannuronic acid (M-blocks), no poly-α-L-guluronate (G-blocks), 12% alternating sequences of β-D-mannuronic acid and α-L-guluronic acid (poly-MG/GM-blocks), 12% MGM-blocks, 172 degrees of polymerization, and di-O-acetylation of M-residues. Interestingly, CMG1418 alginate did not show any cytotoxic or antimetabolic activity. Moreover, compared to algal alginates, CMG1418 alginate exhibited higher and more stable flocculation efficiencies (70-90%) and viscosities (4500-4760 cP) over a wide range of pH and temperatures. Additionally, it displayed soft to flexible gelling abilities and higher water-holding capacities (375%). It also showed thermodynamically more stable emulsifying activities (99-100%) that surpassed the algal alginates and commercial emulsifying agents. However, only divalent and multivalent cations could slightly increase viscosity, gelling, and flocculation. In conclusion, this study explored a structurally di-O-acetylated and poly-G-blocks-deficient, biocompatible alginate, and its pH and thermostable functional properties. This research suggests that CMG1418 alginate is a superior and more reliable substitute for algal alginates in various applications, such as viscosifying, soft gelling, flocculating, emulsifying, and water-holding.