背景:蚊子是许多病原体的媒介,比如疟疾,登革热病毒,黄热病病毒,丝虫和日本脑炎病毒。Wolbachia能够在其宿主中诱导广泛的生殖异常,例如细胞质不相容性。Wolbachia已被提议作为一种工具来修饰对病原体感染具有抗性的蚊子,作为替代的媒介控制策略。这项研究旨在确定海南省不同蚊子种类的天然Wolbachia感染,中国。
方法:使用光陷阱收集成年蚊子,2020年5月至2021年11月,海南省五个地区的人类登陆捕获量和吸气器。根据形态特征鉴定物种,cox1分析的物种特异性PCR和DNA条形码。根据cox1,wsp,16SrRNA和FtsZ基因片段。
结果:对代表15个物种的413只雌性成年蚊子进行了分子鉴定和分析。四种蚊子(白纹伊蚊,库蚊,Albbatus下的Armigeres和Culexgelidus)对Wolbachia感染呈阳性。本研究中测试的所有蚊子的整体Wolbachia感染率为36.1%,但因物种而异。WolbachiaA型,在Ae中检测到B和A×B混合感染。白纹蚊子.共有五种wsp单倍型,从沃尔巴克氏菌感染中检测到6种FtsZ单倍型和6种16SrRNA单倍型。wsp序列的系统发育树分析将它们分为三组(A型,B和C)的Wolbachia菌株与两组的FtsZ和16SrRNA序列进行比较。在Cx中检测到一种新的C型Wolbachia菌株。gelidus由单个基因座wsp基因和三个基因的组合组成。
结论:我们的研究揭示了Wolbachia在海南省蚊子中的流行和分布,中国。了解Wolbachia菌株在当地蚊子种群中的流行和多样性,将为在海南省开展当前和未来基于Wolbachia的媒介控制方法提供所需的部分基线信息。
BACKGROUND: Mosquitoes are vectors of many pathogens, such as malaria, dengue virus, yellow fever virus, filaria and Japanese encephalitis virus. Wolbachia are capable of inducing a wide range of reproductive abnormalities in their hosts, such as cytoplasmic incompatibility. Wolbachia has been proposed as a tool to modify mosquitoes that are resistant to pathogen infection as an alternative vector control strategy. This study aimed to determine natural Wolbachia infections in different mosquito species across Hainan Province, China.
METHODS: Adult mosquitoes were collected using light traps, human landing catches and aspirators in five areas in Hainan Province from May 2020 to November 2021. Species were identified based on morphological characteristics, species-specific PCR and DNA barcoding of cox1 assays. Molecular classification of species and phylogenetic analyses of Wolbachia infections were conducted based on the sequences from PCR products of cox1,
wsp, 16S rRNA and FtsZ gene segments.
RESULTS: A total of 413 female adult mosquitoes representing 15 species were identified molecularly and analyzed. Four mosquito species (Aedes albopictus, Culex quinquefasciatus, Armigeres subalbatus and Culex gelidus) were positive for Wolbachia infection. The overall Wolbachia infection rate for all mosquitoes tested in this study was 36.1% but varied among species. Wolbachia types A, B and mixed infections of A × B were detected in Ae. albopictus mosquitoes. A total of five
wsp haplotypes, six FtsZ haplotypes and six 16S rRNA haplotypes were detected from Wolbachia infections. Phylogenetic tree analysis of
wsp sequences classified them into three groups (type A, B and C) of Wolbachia strains compared to two groups each for FtsZ and 16S rRNA sequences. A novel type C Wolbachia strain was detected in Cx. gelidus by both single locus
wsp gene and the combination of three genes.
CONCLUSIONS: Our study revealed the prevalence and distribution of Wolbachia in mosquitoes from Hainan Province, China. Knowledge of the prevalence and diversity of Wolbachia strains in local mosquito populations will provide part of the baseline information required for current and future Wolbachia-based vector control approaches to be conducted in Hainan Province.