多囊卵巢综合征(PCOS)是育龄期妇女的一种内分泌疾病。目前,PCOS的病理生理学尚不清楚,和有限的治疗选择不能令人满意。初榨椰子油(VCO)是与生殖障碍的药理作用相关的功能性食用油。因此,我们旨在评估VCO是否可以增强雌性大鼠对PCOS的氯米芬(CLO)治疗。大鼠随机分为:(1)对照,(2)PCOS模型,(3)PCOS+CLO,(4)PCOS+VCO,和(5)PCOS+CLO+VCO。PCOS是通过每日来曲唑(1mg/kg,口服)给药21天。PCOS诱导后,CLO,VCO,和CLO+VCO从第22天到第36天施用。血清促性腺激素释放激素(GnRH)水平,卵泡刺激素(FSH),黄体生成素(LH),睾丸激素,雌激素,黄体酮,和催乳素进行了估计。核因子-类红细胞相关因子2(Nrf2)的聚合酶链反应基因表达,血红素加氧酶-1(HO-1),过氧化氢酶(CAT),谷胱甘肽还原酶(GSR),LH受体(LHr),雄激素受体(AR),肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β),和caspase-3进行分析。来曲唑诱导的PCOS引起GnRH显著增加,LH,催乳素,雌激素,和睾丸激素,与对照组相比,FSH显着降低。Nrf2,HO-1,CAT,GSR明显减少,而IL-1β,TNF-α,caspase-3,AR,与对照相比,LHr显著增加。有趣的是,CLO和VCO分别发挥抗炎和内分泌平衡作用。然而,VCO增强LH的CLO效应,催乳素和睾酮,Nrf2,HO-1,CAT,GSR,和AR。VCO可能与CLO协同抑制PCOS中的高雄激素血症和氧化性炎症。
Polycystic ovarian syndrome (PCOS) is an endocrine disorder in women\'s reproductive age. Currently, the pathophysiology of PCOS is unclear, and the limited treatment options are unsatisfactory. Virgin coconut oil (VCO) is functional food oil associated with pharmacological effects in reproductive disorders. Therefore, we aimed to evaluate whether VCO could enhance clomiphene (CLO) therapy against PCOS in female rats. Rats were randomly divided: (1) Control, (2) PCOS model, (3) PCOS + CLO, (4) PCOS + VCO, and (5) PCOS + CLO + VCO. The PCOS was induced via daily letrozole (1 mg/kg, orally) administration for 21 days. After the PCOS induction, CLO, VCO, and CLO + VCO were administered from days 22 to 36. Serum levels of gonadotropin-releasing hormone (GnRH), follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone, estrogen, progesterone, and prolactin were estimated. Polymerase chain reaction gene expression for nuclear factor-erythroid-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), catalase (CAT), glutathione reductase (GSR), LH receptor (LHr), androgen receptor (AR), tumor necrosis factor-alpha (TNF-α), interleukin-1β (IL-1β), and caspase-3 were analyzed. The letrozole-induced PCOS caused considerable increases in GnRH, LH, prolactin, estrogen, and testosterone, whereas FSH decreased significantly compared to the control. The gene expression of Nrf2, HO-1, CAT, and GSR were markedly diminished, while IL-1β, TNF-α, caspase-3, AR, and LHr prominently increased compared to control. Interestingly, the CLO and VCO separately exerted anti-inflammatory and endocrine balance effects. However, VCO-enhanced CLO effect in LH, prolactin and testosterone, Nrf2, HO-1, CAT, GSR, and AR. VCO may synergize with CLO to depress hyperandrogenism and oxidative inflammation in PCOS.