根结线虫病每年在全球农业生产中造成严重的产量和经济损失。DokdonensisMCCC1A00493,一种深海细菌,显示了在体外对南方根结线虫的显着杀线虫活性。然而,有关V.dokdonensisMCCC1A00493的活性物质的信息有限。在这项研究中,通过固相微萃取和气相色谱-质谱法分离和分析了多克顿菌MCCC1A00493中的挥发性有机化合物(VOCs)。四种挥发性有机化合物,即,乙醛,二甲基二硫醚,乙苯,和2-丁酮,被确认,并对其杀线虫活性进行了评价。四种挥发性有机化合物对隐身支原体有多种活性模式。乙醛有直接接触杀灭作用,熏蒸,和吸引活性;二甲基二硫化物具有直接接触杀伤和吸引活性;乙苯具有吸引活性;2-丁酮具有驱除活性。只有乙醛具有熏蒸活性以抑制卵孵化。将这种针对卵和幼体的熏蒸活性结合起来可能是控制M.incognita不同发育阶段的有效策略。直接接触和吸引活动的结合还可以建立针对根结线虫的诱捕和杀死策略。考虑到所有杀线虫的模式或策略,我们可以使用V.dokdonensisMCCC1A00493来建立一个控制根结线虫的综合策略。
Root-knot nematode diseases cause severe yield and economic losses each year in global agricultural production. Virgibacillus dokdonensis MCCC 1A00493, a deep-sea bacterium, shows a significant nematicidal activity against Meloidogyne incognita in vitro. However, information about the active substances of V. dokdonensis MCCC 1A00493 is limited. In this study, volatile organic compounds (VOCs) from V. dokdonensis MCCC 1A00493 were isolated and analyzed through solid-phase microextraction and gas chromatography-mass spectrometry. Four VOCs, namely, acetaldehyde, dimethyl disulfide, ethylbenzene, and 2-butanone, were identified, and their nematicidal activities were evaluated. The four VOCs had a variety of active modes on M. incognita juveniles. Acetaldehyde had direct contact killing, fumigation, and attraction activities; dimethyl disulfide had direct contact killing and attraction activities; ethylbenzene had an attraction activity; and 2-butanone had a repellent activity. Only acetaldehyde had a fumigant activity to inhibit egg hatching. Combining this fumigant activity against eggs and juveniles could be an effective strategy to control the different developmental stages of M. incognita. The combination of direct contact and attraction activities could also establish trapping and killing strategies against root-knot nematodes. Considering all nematicidal modes or strategies, we could use V. dokdonensis MCCC 1A00493 to set up an integrated strategy to control root-knot nematodes.