背景:癌症化疗药物的眼部副作用相对少见。尽管如此,眼部系统对有毒物质具有潜在的高度敏感性。这项研究提出了一个框架来评估长春新碱化疗对眼压的影响,泪液蛋白,与犬传染性性病肿瘤(TVT)的氧化应激。
方法:研究组包括10只TVT犬,他们的诊断是基于细胞学,所有的狗都用长春新碱治疗4周。对每只动物进行完整的眼科检查,随后进行标准Schirmer撕裂试验。在施用长春新碱之前和之后20分钟,用非接触式眼压计测量眼内压(IOP).在提到的任何时候,使用Schirmer测试程序收集泪液样品,并进行蛋白质分析-氧化应激指数(OSI),总抗氧化能力(TAC),总氧化剂状态(TOS),一氧化氮(NO),丙二醛(MDA)测定,并应用标准统计分析。
结果:泪液中的蛋白质没有发现显著差异,但平均注射前和注射后IOP显示每周眼睛显着下降。此外,结果表明氧化应激标志物存在显著差异:OSI增加,NO,MDA,减少TAC。
结论:应认真对待长春新碱治疗患者泪液中氧化应激水平升高的重要性,因为它似乎在眼病的发病机制中起作用。因此,在处方长春新碱前的治疗周,应评估和考虑眼部疾病。
BACKGROUND: The ocular side effects of cancer chemotherapeutic drugs are relatively uncommon. Nonetheless, the ocular system has a potentially high sensitivity to toxic substances. This study proposed a framework to assess the effect of vincristine chemotherapy on intraocular pressure, tear protein, and oxidative stress in canines with transmissible venereal tumor (TVT).
METHODS: The study group comprised 10 dogs with TVT, whose diagnosis was based on cytology, and all dogs were treated with vincristine for 4 weeks. Each animal was given a complete ophthalmic examination, followed by a standard Schirmer tear test. Before and 20 min after administering vincristine, intraocular pressure (IOP) was measured in the eyes with a noncontact tonometer. At any of the times mentioned, tear samples were collected using the Schirmer test procedure and were subjected to protein analysis-oxidative stress index (OSI), total antioxidant capacity (TAC), total oxidant status (TOS), nitric oxide (NO), and malondialdehyde (MDA) were determined, and standard statistical analysis was applied.
RESULTS: No significant differences were found in protein in tears, but mean Pre and Postinjection IOP revealed a significant decrease in the eyes each week. Also, results indicated significant differences in oxidative stress markers: increased OSI, NO, and MDA, and reduced TAC.
CONCLUSIONS: The importance of an increase in oxidative stress levels in the tears of vincristine-treated patients should be taken seriously, as it appears to play a role in the pathogenesis of eye disease. Therefore, during the treatment weeks prior to prescribing vincristine, eye diseases should be evaluated and considered.