Soil metabolites are critical in regulating the dynamics of ecosystem structure and function, particularly in fragile karst ecosystems. Clarification of response of soil metabolism to vegetation succession in karst areas will contribute to the overall understanding and management of karst soils. Here, we investigated the metabolite characteristics of karst soils with different vegetation stages (grassland, brushwood, secondary forest and primary forest) based on untargeted metabolomics. We confirmed that the abundance and composition of soil metabolites altered with vegetation succession. Of the 403 metabolites we found, 157 had significantly varied expression levels across vegetation soils, including mainly lipids and lipid-like molecules, phenylpropanoids and polyketides, organic acids and derivatives. Certain soil metabolites, such as maltotetraose and bifurcose, were sensitive to vegetation succession, increasing significantly from grassland to brushwood and then decreasing dramatically in secondary and primary forests, making them possible indicators of karst vegetation succession. In addition, soil metabolic pathways, such as galactose metabolism and biosynthesis of unsaturated fatty acids, also changed with vegetation succession. This study characterized the soil metabolic profile in different vegetation stages during karst secondary succession, which would provide new insights for the management of karst soils.

UNASSIGNED: The Minqin Oasis, which is located in Wuwei City, Gansu Province, China, faces a very serious land desertification problem, with about 94.5% of its total area desertified. Accordingly, it is crucial to implement ecological restoration policies such as cropland abandonment in this region. In abandoned croplands, abiotic factors such as soil properties may become more important than biotic factors in driving vegetation succession. However, the connections between soil properties and vegetation succession remain unclear. To fill this knowledge gap, this study investigated these connections to explore major factors that affected vegetation succession, which is meaningful to designing management measures to restore these degraded ecosystems.
UNASSIGNED: This study investigated seven 1-29-year-old abandoned croplands using the \"space for time\" method in Minqin Oasis. Vegetation succession was classified into different stages using a canonical correlation analysis (CCA) and two-way indicator species analysis (Twinspan). The link between soil properties and vegetation succession was analyzed using CCA. The primary factors shaping community patterns of vegetation succession were chosen by the \"Forward selection\" in CCA. The responses of dominant species to soil properties were analyzed using generalized additive models (GAMs).
UNASSIGNED: Dominant species turnover occurred obviously after cropland abandonment. Vegetation succession can be classified into three stages (i.e., early, intermediate, and late successional stages) with markedly different community composition and diversity. The main drivers of vegetation succession among soil properties were soil salinity and saturated soil water content and they had led to different responses of the dominant species in early and late successional stages. During the development of vegetation succession, community composition became simpler, and species diversity decreased significantly, which was a type of regressive succession. Therefore, measures should be adopted to manage these degraded, abandoned croplands.

Soil cover is a prevailing method used at mine sites to ensure the safety of hazardous materials and restore ecological functions when the base materials are unfavorable for plant growth. The former open-pit Ningyo-toge Mine was backfilled with overburden and neutralized smelting residues and covered with soil in 1987. After 36 years, the vegetation remained dominated by the perennial herb Miscanthus sinensis, and woody plant establishment did not progress successfully. This study investigated the factors that inhibited woody plant establishment at the site. The soil profile survey revealed that the soil cover formed Bg horizons (pseudogley soil) with cloudy mottling, representative of poorly drained soil. In the Bg horizon, woody plant roots of Pinus densiflora and Weigela hortensis exhibited growth inhibition. Elemental analysis revealed that in the Bg horizon the roots of P. densiflora and W. hortensis accumulated high Fe concentrations exceeding 10,000 mg/kg DW at critical levels. Our results suggested that woody plant roots in the Bg horizon may have suffered from chronic oxygen deficiency accompanied by excessive Fe stress in the soil cover. Topsoil water content (<50 mm) and microtopographic features were not critical factors disrupting woody plant establishment because some individuals were growing in areas with high soil water content, exceeding 60%. Considering that woody plant roots were developed primarily in the shallow A horizon, A horizon formation by M. sinensis is a key step in initiating woody plant establishment by improving the soil structure and physiochemical characteristics of the soil cover, such as carbon content, exchangeable nutrients, and air-filled porosity. For successful mine pollution control and vegetation recovery, implementing an appropriate topsoil system, such as native forest soil, loosely graded and minor infiltration layer above the overburden would be necessary.

Extensive unforested sandy areas on the margins of floodplains and riverbeds, formed by dunes, barchans, and accumulation berms, are a ubiquitous feature across northern Eurasia and Alaska. These dynamic landscapes, which bear witness to the complex Holocene and modern climatic fluctuations, provide a unique opportunity to study ecosystem evolution. Within this heterogeneous assemblage, active dunes, characterized by their very sparse plant communities, contrast sharply with the surrounding taiga (boreal) forests common for the stabilized dunes. This juxtaposition makes these regions to natural laboratories to study vegetation succession and soil development. Through a comprehensive analysis of climate, geomorphology, vegetation, soil properties, and microbiome composition, we elucidate the intricacies of cyclic and linear ecosystem evolution within a representative sandy area located along the lower Nadym River in Siberia, approximately 100 km south of the Arctic Circle. The shift in the Holocene wind regime and the slow development of vegetation under harsh climatic conditions promoted cyclical ecosystem dynamics that precluded the attainment of a steady state. This cyclical trajectory is exemplified by Arenosols, characterized by extremely sparse vegetation and undifferentiated horizons. Conversely, accelerated vegetation growth within wind-protected enclaves on marginally stabilized soils facilitated sand stabilization and subsequent pedogenesis towards Podzols. Based on soil acidification due to litter input (mainly needles, lichens, and mosses) and the succession of microbial communities, we investigated constraints on carbon and nutrient availability during the initial stages of pedogenesis. In summary, the comprehensive study of initial ecosystem development on sand dunes within taiga forests has facilitated the elucidation of both common phases and spatiotemporal dynamics of vegetation and soil succession. This analysis has further clarified the existence of both cyclic and linear trajectories within the successional processes of ecosystem evolution.

We investigated the changes of soil nutrients and plant communities in the artificial sand fixation forests of Caragana korshinskii with different ages. The results showed that soil organic carbon and soil total nitrogen contents increased with the stand ages, and were significantly higher in 40 and 50 year-old than other ages. Soil organic carbon and total nitrogen contents recovered much faster in the surface layer (0-10 cm) than in others. Soil nutrient stoichiometric ratios (C:P, N:P) in the 0-10 cm soil layer differed significantly among different stand ages. With the increases of stand age, C and N contents in C. korshinskii leaves increased significantly, and reached the maximum at 50 year-old. Leaf P content increased first and then decreased, being maximum at 18 year-old. Leaf C:N first increased and then decreased, being maximum at 12 year-old. The contents of photosynthetic pigments and leaf C:P and N:P decreased first and then increased, being minimum at 18 year-old. C. korshinskii was mainly influenced by N availability before 40 year-old, but mainly limited by P after. The species number, density, and vegetation cover of annual and perennial herbaceous plants increased with stand ages, and the community shifted from a simple shrub plant community to a complex shrub-herb community. The biomass of C. korshinskii and herbaceous plants increased significantly with stand age, and had a significant positive correlation with the contents of soil organic carbon, total nitrogen and N:P.
以晋西北丘陵风沙区不同林龄(6、12、18、40和50年)柠条锦鸡儿人工林为研究对象,分析其土壤养分和植物群落变化特征。结果表明: 土壤有机碳和全氮随着林龄的增加而增加,在40和50年时养分含量显著高于其他林龄,且土壤表层(0～10 cm)有机碳和土壤全氮恢复远快于其他土层,表层土壤养分计量比C∶P、N∶P在不同林龄间存在显著差异。随着林龄的增加,柠条锦鸡儿叶片C、N含量显著增加,在50年时达到最大值;叶片P含量先增加后减少,18年时达到最大值;叶片C∶N先增加后减少,12年时达到最大值;而光合色素含量、C∶P、N∶P均先减少后增加,18年时为最小值。林龄小于40年的柠条锦鸡儿主要受土壤N的影响,大于40年主要受P限制。柠条锦鸡儿林下一年生和多年生草本植物种类数量、密度、盖度均随林龄增加而增加,植物群落逐渐由简单的灌木向复杂的灌草群落演变。柠条锦鸡儿与草本植物生物量随着林龄的增加显著增加,且均与土壤有机碳、全氮含量和N∶P呈显著正相关关系。.

Wetland vegetation plays a crucial role in wetland conservation policy formulation and global climate change research. This study analyzed remotely sensed images of West Dongting Lake (DTL) Wetland from 1994 to 2020. This wetland is one of the most important wetlands in the world. At the pixel scale, we applied the histogram comparison approach, the range variability analysis (RVA) method, and the structural equation model (SEM) to quantify spatial changes in the hydrological conditions of wetland lakes and the ecological effects of environmental factors (precipitation, temperature, nutrients, water coverage) on vegetation. We propose a climate (C) - hydrological status (S) - vegetation response (R) (CSR) framework to elucidate the propagation relationships between climate, hydrology, and wetland vegetation conditions. The study found that the hydrological degradation promotes the succession of vegetation into the lake, and the distribution is concentrated in the northern Yangtze River inflow area. And the extent of hydrological changes in the West DTL region reached 34.5% during the flood period. In addition, the post-dam period showed a high degree of hydro-ecological failure, accounting for 65% of the total. Within the wetland area, there was a significant negative correlation between water coverage nutrient levels and bare vegetation within the lake area. Nutrient levels were also significantly negatively correlated with wetland vegetation conditions. Rainfall and temperature influence wetland vegetation by affecting the condition of the water body. This research provides valuable insights into managing wetland water resources and ecological restoration under the influence of climate change and human activities and provides a basis for decision-making.

Microbial necromass carbon (MNC) is a stable part of SOC that makes up most of the C pool in land ecosystem. However, the contribution of MNC to SOC accumulation during afforestation is still unclear, particularly in the deep soil. Based on the collection and biomarker analysis of the forest succession sequence and soil profiles with significant depth on the Loess Plateau located China, we study the vertical distribution characteristics and control of MNC. The results found that MNC content increased with succession and decreased with soil depth. On average, the MNC content of a climax forest was 2.23 times higher than that of farmland. The FNC:BNC ratio increased with vegetation succession and decreased with soil depth. Although the MNC content decreased with soil depth, the necromass accumulation coefficient increased. The contribution of MNC to SOC in deep soil (60-100 cm) of pioneer forest was more than 10 % higher than that of farmland, suggesting that afforestation had a relatively positive effect on MNC stabilization and accumulation in deep soils. The microbial biomass and soil nutrient characteristics (i.e., TN, SOC, DOC, and DON) are important factors in mediating the accumulation of MNC in the succession of farmland to forest. These findings demonstrate the potential of MNC in deep soil and provide scientific guidance for sustainable reforestation management based on the carbon pump theory at regional scales.

Understanding heavy metals (HMs) accumulation and transportation is the foundation to assess the ecological risks caused by the pollution of HMs in terrestrial ecosystems. There are large knowledge gaps regarding impacts of vegetation succession on shaping the HMs accumulation, transportation and allocation in the remote alpine regions. Herein, we comprehensively investigated the distribution and source contribution of mercury (Hg), cadmium (Cd) and chromium (Cr) along with vegetation succession in a deglaciated forest chronosequence of Qinghai-Tibet Plateau. Results showed that Hg and Cd were highly enriched in organic soils, while Cr concentrations and pool sizes decreased significantly with the vegetation succession. Atmospheric Hg deposition contributed to the dominant Hg sources in topsoil (74 - 87%), whereas moraine weathering was the main source of Cr (73 - 76%). Both moraine (18 - 48%) and atmospheric deposition inputs (52 - 82%) affected Cd accumulation in topsoil. Over the last century, the accumulation rate of Hg and Cd showed the distinctly decreasing trends due to the vegetation leading to the elevated atmospheric depositions at the earlier deglacial sites. The negative accumulation rate of Cr along with the vegetation succession reflected the formation of organic soil diluting the geogenic inputs of Cr.

Large-scale afforestation is considered a natural way to address climate challenges (e.g., the greenhouse effect). However, there is a paucity of evidence linking plant diversity to soil carbon sequestration pathways during long-term natural restoration of temperate vegetation. In particular, the carbon sequestration mechanisms and functions of woody plants require further study. Therefore, we conducted a comparative study of plant diversity and soil carbon sequestration characteristics during 150 years of natural vegetation restoration in the temperate zone to provide a comprehensive assessment of the effects of long-term natural vegetation restoration processes on soil organic carbon stocks. The results suggested positive effects of woody plant diversity on carbon sequestration. In addition, fine root biomass and deadfall accumulation were significantly positively correlated with soil organic carbon stocks, and carbon was stored in large grain size aggregates (1-5 mm). Meanwhile, the diversity of Fabaceae and Rosaceae was observed to be important for soil organic carbon accumulation, and the carbon sequestration function of shrubs should not be neglected during vegetation restoration. Finally, we identified three plants that showed high potential for carbon sequestration: Lespedeza bicolor, Sophora davidii, and Cotoneaster multiflorus, which should be considered for inclusion in the construction of local artificial vegetation. Among them, L. bicolor is probably the best choice.

Moso bamboo (Phyllostachys edulis) is China\'s most important economic bamboo species. With a continuous decline in the value of its shoots and timber and an increase in affiliated labor and production costs, many of these stands have been abandoned, resulting in the occurrence of vegetation succession. Currently, our understanding on changes in soil microbial stoichiometric and entropic effects and associated imbalances following stand abandonment is limited. Accordingly, this study explores three timescales of Ph. edulis stand abandonment (i.e., 0, 9, and 21 years) to investigate soil-microbial carbon (C), nitrogen (N), and phosphorus (P) dynamics within a 30 cm soil profile. Results showed that (1) following abandonment, vegetation succession significantly influenced soil carbon (Csoil), nitrogen (Nsoil), and phosphorus (Psoil), microbial biomass (Cmic), nitrogen (Nmic), and phosphorus (Pmic), and Csoil:Nsoil:Psoil and Cmic:Nmic:Pmic ratios. Additionally, Csoil, Nsoil, Psoil, Cmic, Nmic, Pmic all increased significantly over time following abandonment. Moreover, Csoil:Nsoil, Cmic:Pmic, and Nmic:Pmic ratios clearly increased while Csoil:Psoil, Nsoil:Psoil, and Cmic:Nmic ratios all significantly decreased. (2) Soil microbial entropy nitrogen (qMBN) and soil microbial imbalances in Cimb:Nimb increased while soil microbial entropy carbon (qMBC), soil microbial entropy phosphorus (qMBP), and soil microbial imbalances in Cimb:Pimb and Nimb:Pimb decreased over time following abandonment. (3) Redundancy analysis (RDA) indicated that Csoil:Nsoil and Cmic:Pmic ratios were key influencing factors of microbial quotient (qMB), explaining 55.35 % and 24.39 % of variation, respectively. Following abandonment, positive or negative successional impacts on Csoil:Nsoil:Psoil, microbial C, N, P stoichiometric imbalances (Cimb:Nimb:Pimb), and Csoil:Nsoil:Psoil ratios had a positive effect on qMB. Collectively, these findings highlight the importance of Csoil:Nsoil:Psoil and Cimb:Nimb:Pimb ratios in regulating qMB induced by vegetation succession following Ph. edulis abandonment, and provide valuable information for vegetation restoration and establishment of bamboo mixed forest.