目的:评价辅酶Q10(CoQ10)对牙槽骨改建和正畸牙齿移动(OTM)的影响。
方法:将42只雌性Sprague-Dawley大鼠放置正畸矫治器,分为两组:正畸力(OF)组(n=21)和OFCoQ10(CoQ10)治疗组(n=21)。每组分为3个亚组,在第3、7和14天处死大鼠。CoQ10和OF组的大鼠给予100mg/kgb.w./天CoQ10(在1mL/b.w.大豆油中)和1mLb.w./天大豆油,分别,通过口胃灌胃。在实验结束时测量OTM。破骨细胞,成骨细胞和毛细血管数量;血管内皮生长因子(VEGF),受体激活剂核κB配体(RANKL)和组织中的骨保护素(OPG)水平;确定血液中的总抗氧化剂状态(TAS)和总氧化剂状态(TOS)。
结果:与OF组相比,CoQ10治疗组显示正畸牙齿移动和破骨细胞和毛细血管数量减少。的确,VEGF和RANKL水平下降,而OPG的水平增加,除了第7天。此外,CoQ10治疗组在第7天和第14天表现出更低的TOS和更高的TAS(p<0.05)。组织学结果显示,CoQ10组成骨细胞形态发生改变;各组间成骨细胞数量差异无统计学意义(p>0.05)。
结论:由于其对氧化应激和炎症的影响,CoQ10通过抑制破骨细胞分化调节骨重建,促进成骨细胞分化和减少OTM的量。
结论:考虑到OTM可能会随着CoQ10的使用而减慢,诸如正畸治疗持续时间之类的主题,治疗计划中应考虑正畸力激活和预约频率。据预测,CoQ10的使用将支持临床应用中的治疗有效性,例如通过调节骨骼调节和抑制/优化不需要的牙齿移动的锚固方法来预防正畸治疗中的复发。
OBJECTIVE: To evaluate the effects of coenzyme Q10 (CoQ10) on alveolar bone remodeling and orthodontic tooth movement (OTM).
METHODS: An orthodontic appliance was placed in 42 female Sprague‒Dawley rats were divided into two groups: the orthodontic force (OF) group (n = 21) and the OF + CoQ10 (CoQ10) treatment group (n = 21). Each group was divided into 3 subgroups, and the rats were sacrificed on days 3, 7 and 14. The rats in CoQ10 and OF groups were administered 100 mg/kg b.w./day CoQ10 (in 1 mL/b.w. soybean oil) and 1 mL b.w./day soybean oil, respectively, by orogastric gavage. The OTM was measured at the end of the experiment. The osteoclast, osteoblast and capillary numbers; vascular endothelial growth factor (VEGF), receptor activator nuclear kappa B ligand (RANKL) and osteoprotegrin (OPG) levels in tissue; and total antioxidant status (TAS) and total oxidant status (TOS) in blood were determined.
RESULTS: Compared with the OF group, the CoQ10 treatment group exhibited decreased orthodontic tooth movement and osteoclast and capillary numbers. Indeed, the levels of VEGF and RANKL decreased, while the levels of OPG increased except on day 7. Additionally, the CoQ10 treatment group exhibited lower TOS and higher TAS on days 7 and 14 (p < 0.05). Histological findings showed that the morphology of osteoblasts changed in the CoQ10 group; however, there was no significant difference in the number of osteoblasts between the groups (p > 0.05).
CONCLUSIONS: Due to its effect on oxidative stress and inflammation, CoQ10 regulates bone remodeling by inhibiting osteoclast differentiation, promoting osteoblast differentiation and reducing the amount of OTM.
CONCLUSIONS: Considering that OTM may be slowed with the use of CoQ10, topics such as orthodontic treatment duration, orthodontic force activation and appointment frequency should be considered in treatment planning. It is predicted that the use of CoQ10 will support the effectiveness of treatment in clinical applications such as preventing relapse in orthodontic treatment by regulating bone modulation and anchorage methods that suppress/optimize unwanted tooth movement.