简介:目前正在研究维生素D状态的新标志物,包括游离的25-(OH)D(25-(OH)DF)和维生素D代谢物比率(24,25-(OH)2D3:25-(OH)D3;VMR)。VMR可以提供关于运动员维生素D代谢的额外功能信息。因此,本研究的主要目的是评估25-(OH)DF,生物可利用性25-(OH)D(25-(OH)DB),VMR,以及半个赛季不同训练期间的心理生理应激指标。第二个目的是评估维生素D结合蛋白(VDBP)之间的关联,总和自由25-(OH)D,VMR,和职业足球运动员的心理物理压力标记。此外,我们研究了25-(OH)D3和维生素D代谢物(24,25-(OH)2D3,3-epi-25-(OH)D3)之间的关系,以确定不同训练时期的训练负荷是否影响维生素D代谢组.方法:在半年的六个不同时间点(V1-6月;V2-7月;V3-8月;V4-10月;V5-12月;V6-1月)对20名职业足球运动员进行了测试。结果:分析表明VDBP有明显的季节性节律,和总计25-(OH)D(25-(OH)DT),25-(OH)DB,24,25-(OH)2D3、3-epi-25-(OH)D3、25-(OH)D3:24,25-(OH)2D3和24,25-(OH)2D3:25-(OH)D3VMR。在25-(OH)DT之间没有检测到相关性,25-(OH)DB,25-(OH)DF,维生素D代谢物,VMR,VDBP,和铁蛋白,肝酶(天冬氨酸转氨酶[AST]和丙氨酸转氨酶[ALT]),肌酸激酶(CK),皮质醇,睾丸激素,和睾酮与皮质醇比(T/C)在每个时期(V1-V6)。然而,在每个训练期间,25-(OH)D3和24,25-(OH)D3之间存在很强的统计学显着相关性。结论:总之,VDBP存在季节性节奏,25-(OH)DT,25-(OH)DB,维生素D代谢物(24,25-(OH)2D3,3-epi-25-(OH)D3),和VMR(25-(OH)D3:24,25-(OH)2D3,25-(OH)D3:3-表-25-(OH)D3)。然而,没有检测到25-(OH)DF和心理应激标志物的节律(铁蛋白,肝酶,CK,睾丸激素,皮质醇,和T/C比)。此外,游离和总25-(OH)D与心理物理应激标志物之间的关系并未证明游离优于总测量值。此外,不同训练时期的训练负荷不会影响足球运动员静息状态下维生素D代谢产物的浓度。
Introduction: Novel markers of vitamin D status are currently being investigated, including free 25-(OH)D (25-(OH)DF) and the vitamin D metabolite ratio (24,25-(OH)2D3:25-(OH)D3;
VMR). The
VMR may provide additional functional information on vitamin D metabolism in athletes. Therefore, the main objective of the current study was to evaluate 25-(OH)DF, bioavailable 25-(OH)D (25-(OH)DB),
VMR, and psychophysical stress markers during different training periods over a half-season. The second aim was to assess the association between vitamin D binding protein (VDBP), total and free 25-(OH)D, VMRs, and psychophysical stress markers in professional football players. Moreover, we examined the relationship between 25-(OH)D3 and vitamin D metabolites (24,25-(OH)2D3, 3-epi-25-(OH)D3) to determine if training loads in different training periods influenced the vitamin D metabolome. Methods: Twenty professional football players were tested at six different time points across half a year (V1-June; V2-July; V3-August; V4-October; V5-December; V6-January). Results: Analyses indicated a significant seasonal rhythm for VDBP, and total 25-(OH)D (25-(OH)DT), 25-(OH)DB, 24,25-(OH)2D3, 3-epi-25-(OH)D3, 25-(OH)D3:24,25-(OH)2D3, and 24,25-(OH)2D3:25-(OH)D3 VMRs throughout the training period. No correlation was detected between 25-(OH)DT, 25-(OH)DB, 25-(OH)DF, vitamin D metabolites, VMRs, VDBP, and ferritin, liver enzymes (aspartate transaminase [AST] and alanine transaminase [ALT]), creatine kinase (CK), cortisol, testosterone, and testosterone-to-cortisol ratio (T/C) in each period (V1-V6). However, there was a strong statistically significant correlation between 25-(OH)D3 and 24,25-(OH)D3 in each training period. Conclusion: In conclusion, a seasonal rhythm was present for VDBP, 25-(OH)DT, 25-(OH)DB, vitamin D metabolites (24,25-(OH)2D3, 3-epi-25-(OH)D3), and VMRs (25-(OH)D3:24,25-(OH)2D3, 25-(OH)D3:3-epi-25-(OH)D3). However, no rhythm was detected for 25-(OH)DF and markers of psychophysical stress (ferritin, liver enzymes, CK, testosterone, cortisol, and T/C ratio). Moreover, the relationships between free and total 25-(OH)D with psychophysical stress markers did not demonstrate the superiority of free over total measurements. Furthermore, training loads in different training periods did not affect resting vitamin D metabolite concentrations in football players.